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There are no guidelines as to the safe limit of the perforator flap. We make an attempt to define the safe limit of local perforator flap for lower limb reconstruction by comparing it with the limb length of the patient, which is a constant variable for a particular patient.35 below knee perforator flaps were included in the study and were based on a single islanded perforator. The maximum flap length from the perforator was compared to the limb length (from lateral malleolus to head of fibula). Using EPI info 6.04 D software, analysis of the data was done. Perforator propeller flaps with...

Microarray is a novel technology to identify gene expression of thousands of genes simultaneously. This work is attempted to perform microarray data analyses to determine differential gene expression using the open-source R programming environment in conjunction with the open-source Bioconductor software.We describe procedures for analysis of data using box plots and recommended procedures from Affymetrix for quality control are discussed. The Robust Multichip Averaging (RMA) and MAS5 procedure was used for background correction, normalization and summarization of the AffyBatch probe-level...