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Microarray is a novel technology to identify gene expression of thousands of genes simultaneously. This work is attempted to perform microarray data analyses to determine differential gene expression using the open-source R programming environment in conjunction with the open-source Bioconductor software.We describe procedures for analysis of data using box plots and recommended procedures from Affymetrix for quality control are discussed. The Robust Multichip Averaging (RMA) and MAS5 procedure was used for background correction, normalization and summarization of the AffyBatch probe-level...