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This book deals with cloning of maltase gene of Bacillus licheniformis in Escherichia coli. During the course of study, genomic DNA isolation from Bacillus licheniformis strain ATCC 14580 has been followed by the amplification of maltase gene using a pair of specific forward and reverse primers under optimized PCR parameters. The DNA isolated from Bacillus licheniformis was used as a template and followed amplification, PCR product was purified using Novagen gene kit. T4 DNA ligase was used to ligate the purified PCR product with cloning vector pTZ57R/T. Escherichia coli strain DH5 was used...

It is the need of the day to introduce strategies towards conservation of genetic resources of livestock in several of its forms. Information gathered there of from various sources viz socio-economic knowledge, indigenous understanding and circumstances, production system, phenotypic, genotypic and molecular studies can be utilized in a fruitful and productive manner to support and hold up steps for conservation strategies. Morphological and genetic characterization of native sheep breeds is of immense importance that is achievable by understanding and investigating the genetic associations...