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wyszukanych pozycji: 7
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Cloning and expression
ISBN: 9783639300604 / Angielski / Miękka / 2011 / 112 str. Termin realizacji zamówienia: ok. 10-14 dni roboczych. In earlier days, production of industrially important enzymes was carried out by conventional methods of optimizing cultural conditions in flasks and fermentors. With passage of time new techniques are being developed like cloning. In cloning specific genes are amplified and ligated in specific plasmid that is in turn transformed into the host cells. In this book cloning and expression of xylanase gene of Bacillus licheniformis is discussed. pTZ57R/T and pET22b plasmids are used to clone xylanase gene. Reasonable amount of xylanase is obtained. By using this technique large amount of enzyme...
In earlier days, production of industrially important enzymes was carried out by conventional methods of optimizing cultural conditions in flasks and ...
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cena:
225,35 zł |
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Effect of RNA interference and Microarray
ISBN: 9783639242188 / Angielski / Miękka / 2010 / 140 str. Termin realizacji zamówienia: ok. 10-14 dni roboczych. Over the last decade, the applications of
RNAi and microarray have increased exponentially, and are being applied to explore the expression profile of whole genome (particularly genes coding for proteins). Very little literature exists regarding RNAi against non-coding RNA (hot stuff nowadays) and the expression profile of non-coding RNAs. This book therefore, provide a systematic approach of targeting all types of non-coding RNAs (snoRNAs, snRNAs, SRP and Y RNA) by RNAi and expression ... Over the last decade, the applications of
RNAi and microarray have increased exponentially, and are being applied to ... |
cena:
271,34 zł |
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miroRNAs target sites
ISBN: 9783639334340 / Angielski / Miękka / 2011 / 132 str. Termin realizacji zamówienia: ok. 10-14 dni roboczych. Since the discovery of microRNAs (miRNAs) their role has now been established in translational repression and gene silencing. In metazoans the miRNA target sites are in the three prime untranslated regions (3'UTR) of the mRNA. The function of miRNAs appears to be in gene regulation. Several miRNAs have been found to have links with some types of cancers, heart diseases, nervous system etc. In this book specific sites targeted by miRNAs in the 3 UTRs of mRNAs in the genome of C. briggsae were identified with the help of thermodynamic studies. RNAhybrid software was also utilized in the...
Since the discovery of microRNAs (miRNAs) their role has now been established in translational repression and gene silencing. In metazoans the miRNA t...
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cena:
271,34 zł |
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Cloning
ISBN: 9783639296310 / Angielski / Miękka / 2010 / 80 str. Termin realizacji zamówienia: ok. 10-14 dni roboczych. In earlier days, production of industrially important enzymes was carried out by conventional methods of optimizing cultural conditions in flasks and fermentors. With passage of time new techniques are being developed like cloning. In cloning specific genes are amplified and ligated in specific plasmid that is in turn transformed into the host cells. In this book cloning and expression of alpha amylase gene of Bacillus licheniformis is discussed. pTZ57R/T and pET22b plasmids are used to clone amylase gene. Reasonable amount of amylase is obtained. By using this technique large amount of...
In earlier days, production of industrially important enzymes was carried out by conventional methods of optimizing cultural conditions in flasks and ...
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cena:
236,62 zł |
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Motifs of untranslated regions in C. elegans and C. briggsea
ISBN: 9783846500385 / Angielski / Miękka / 60 str. Termin realizacji zamówienia: ok. 10-14 dni roboczych. Untranslated regions (UTRs) are important regions in genes that are situated at 3 and 5 ends and are involved in the regulation of gene expression. UTRs on 3 end acts as complementary sequences for binding of microRNAs. The length and sequence of UTRs varies from species to species. In this work various conserved motifs in the untranslated regions of the genes have been reported by using software tool. Very little literature is available on the motif studies in the UTRs. This book therefore provides the insight on the presence of motifs with repeated single nucleotide in the 3 and 5 UTRs....
Untranslated regions (UTRs) are important regions in genes that are situated at 3 and 5 ends and are involved in the regulation of gene expression. UT...
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cena:
225,35 zł |
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Bacitracin: : Production and purification of Zn-bacitracin
ISBN: 9783639264180 / Angielski / Miękka / 2010 / 172 str. Termin realizacji zamówienia: ok. 10-14 dni roboczych. In the past 50 years, numerous polypeptide antibiotics have been identified mainly by bacterial production. These polypeptide antibiotics have been shown to have antibacterial activity against many bacteria specially gram positive bacteria. Some of these polypeptide antibiotics could also be used as growth promoter in poultry feed. This book also provides detailed information about production of bacitracin by Bacillus licheniformis. In this regard, systematic approach was adopted. In the first step, different isolates of Bacillus licheniformis were isolated from soil, milk and poultry...
In the past 50 years, numerous polypeptide antibiotics have been identified mainly by bacterial production. These polypeptide antibiotics have been sh...
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cena:
312,73 zł |
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Cloning of Maltase Gene of Bacillus Licheniformis in Escherichia Coli
ISBN: 9783659104015 / Angielski / Miękka / 2012 / 72 str. Termin realizacji zamówienia: ok. 10-14 dni roboczych. This book deals with cloning of maltase gene of Bacillus licheniformis in Escherichia coli. During the course of study, genomic DNA isolation from Bacillus licheniformis strain ATCC 14580 has been followed by the amplification of maltase gene using a pair of specific forward and reverse primers under optimized PCR parameters. The DNA isolated from Bacillus licheniformis was used as a template and followed amplification, PCR product was purified using Novagen gene kit. T4 DNA ligase was used to ligate the purified PCR product with cloning vector pTZ57R/T. Escherichia coli strain DH5 was used...
This book deals with cloning of maltase gene of Bacillus licheniformis in Escherichia coli. During the course of study, genomic DNA isolation from Bac...
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cena:
225,35 zł |
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