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In the history of leishmaniasis, several
methods were used for classification, characterization and
identification of the infecting parasites. The conventional
methods of clinical diagnosis of CL have ranged from
clinical picture and epidemiological data, visualizing the
amastigotes by microscopy of stained smears from skin touch
specimens or biopsies to in-vitro culturing of the parasite.
These conventional methods are, however, limited in
sensitivity, need an experienced hand and do not...

In this study the role of epicuticular wax in transmission of CLCuV has been studied. Epicuticular waxes plays very important role in hindering the attachment of insects to the leaves. In this study the wax deficient mutant plants GaWM3 were used as experimental plants while Gossypium arboreum (786) were used as negatively controlled plants as it is reported that whitefly can not transmit virus in it and G. hirsutum (MNH-93) were taken as negative and positive control plants because it has severe viral attack in past. It is important to mention here that GaWM3 were produced in our laboratory...