PCR Protocols in Molecular Toxicology

ISBN-13: 9780849333446 / Angielski / Miękka / 1997 / 252 str.

John P. Vanden Heuvel; John P. Vanden Heuvel

Opis

Molecular toxicology is an emerging discipline that utilizes molecular and cell biology to understand how drugs and chemicals result in their unwanted effects. PCR Protocols in Molecular Toxicology is a practical guide to the use of polymerase chain reaction (PCR) to help examine, on a molecular and cellular level, how toxic responses are manifested. It offers a basic understanding of PCR and its optimization, as well as describing specific, high-impact areas of molecular toxicology and recent advances. The following techniques are described in detail:

Quantitative reverse transcriptase PCR and methods to examine gene expression

Differential display cloning

Cloning and library screening by PCR

Genotype and polymorphism analysis of drug and toxicant metabolizing enzymes

Basic, non-PCR based molecular biology methods
PCR Protocols in Molecular Toxicology will aid both novices and experienced PCR practitioners in using PCR to its fullest potential.

Spis treści

Chapter 1. The Basics of the Polymerase Chain Reaction -- /William B. Mattes -- Chapter 2. Analysis of Gene Expression -- /John P. Vanden Heuoel -- Chapter 3. Differential Display PCR -- /J. Christopher Corton -- Chapter 4 . Cloning by PCR -- /John P. Vanden Heuuel -- Chapter 5. Genotype Analysis -- /Douglas A. Bell and Gary Pittman -- Chapter 6 . General Molecular Biology Techniques -- /John P. Vanden Heuvel -- Appendix I — List of Suppliers -- Appendix II — Companies, Online Catalogs, and Internet Resources -- Index.

O autorze

Vanden Heuvel, John P.