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Neonatal Cholestasis (NC) caused by a wide range of conditions, among these causes biliary atresia (BA) is the commonest . Timely differentiation of BA from other causes of NC was a challenge to clinician as the former need urgent lifesaving surgery while the later might only need medical treatment for recovery. Carnitine, acyl carnitines and amino acids metabolism has an ultimate relation to liver status. These metabolomics could be detected by high performance liquid chromatography tandem mass spectrometry (HPLC MS/MS) and their levels could give an insight about differential expression and...