Trace and Ultratrace Analysis by HPLC

ISBN-13: 9780471514190 / Angielski / Twarda / 1992 / 432 str.

Satinder Ahuja

Opis

For the analyses of DNA and RNA, high pressure/performance liquid chromatography (HPLC) is proving extremely useful as trace and ultratrace analyses can provide potentially helpful data for diagnostic studies. This text deals with both analyses, executed at or below ppm level, and with on-column injection at low microgram or nanogram level. A variety of applications, studies and methods are used as focal points for discussion, along with theoretical and equipment considerations.

Spis treści

The Scope of Trace and Ultratrace Analysis by HPLC.

Theoretical Considerations.

Equipment Considerations.

Sensitive Detectors in HPLC.

Sample Preparation.

Method Development for Trace and Ultratrace Analysis.

Selectivity Optimization for Trace and Ultratrace Analysis.

Optimizing Detectability.

Applications.

Index.

O autorze

About the author SATINDER AHUJA is Senior Research Fellow in the Physical and Analytical Chemistry Division of CIBA–Geigy Corporation. Dr. Ahuja, who enjoys an international reputation in his field, is a member of the American Chemical Society, IUPAC and AAPS, and the editor/author of five books, including Ultratrace Analysis of Pharmaceuticals and Other Compounds of Interest (Wiley, 1986) and Selectivity and Detectability Optimizations in HPLC (Wiley, 1989). His publications and presentations include basic investigations on various modes of chromatography, automation/computerization, and analytical evaluations of drug candidates. Dr. Ahuja earned his PhD in pharmaceutical analytical chemistry at the Philadelphia College of Pharmacy and Science.

Blurb

High Performance Liquid Chromatography in Biotechnology Edited By William S. Hancock Analytical chemists, biochemists, or chemical engineers will find this up–to–date guide to HPLC’s recent developments essential to enhancing their on–the–job technical expertise. Extensive coverage includes the broad applications of HPLC, ranging from major chromatographic techniques (including reversed phase, ion exchange, affinity and hydrophobic interaction chromatography) to specific separations such as in monoclonal antibody and nucleic acid purification. The book also highlights the techniques required for a quality control program and such advanced technology as mass spectrometry. 1990 (0 471–82584–0) 576 pp. Unified Separation Science J. Calvin Giddings This advanced text/monograph brings together, for the first time in a single volume, the variety of techniques used for chemical separations by outlining their common underlying mechanisms. The mass transport phenomena underlying all separation processes are developed in a simple physicalmathematical form, facilitating analysis of alternative separation techniques and the factors integral to separation power. The first six chapters provide generic background material applicable to a wide range of separation methods, including the theoretical foundations of separations rooted in transport, flow, and equilibrium phenomena. The final five chapters illustrate specific techniques and methods, including electrophoretic and sedimentation techniques, field–flow fractionation, and chromatography. 1991 (0 471–52089–6) 352 pp.