Single Cell Diagnostics: Methods and Protocols

ISBN-13: 9781617376542 / Angielski / Miękka / 2010 / 182 str.

Alan Thornhill

Opis

Single Cell Diagnostics: Methods and Protocols applies modern
molecular diagnostic techniques to the analysis of single cells, small
numbers of cells, or cell extracts. Emphasis is placed on non-invasive
analysis of single cell metabolites and the direct analysis of RNA and
DNA from single cells, with a focus on polymerase chain reaction and
fluorescence in situ hybridization. The authors, all leading
scientists in the field, provide current and innovative methods for
extracting useful, accurate, and reliable data from single-cell
samples. The stepwise, technically precise protocols presented in this
volume thoroughly describe single-cell assays, from material collection
and isolation of cells to data interpretation. Cell-types analyzed
include embryonic blastomeres, fibroblasts, and lymphocytes. Among the
techniques described are real-time quantitative PCR, isothermal whole
genome amplification, comparative genomic hybridization, real-time
genetic expression analysis, and the production of RNA and cDNA
libraries. This handbook will be essential for researchers and
clinicians, and practitioners providing care for couples seeking
treatment for infertility or preimplantation genetic diagnosis.

Recenzje

From the reviews:

"Single cell diagnostics has become an increasingly important field for both clinicians and researchers who are involved in infertility treatment. ... For researchers this book provides not only detailed state of the art protocols for single cell diagnostics, but also valuable notes on troubleshooting and pitfalls. For clinicians in the field of infertility this nice overview gives an insight into single cell diagnostics and its scientific background. ... This book can be warmly recommended to clinicians and researchers caring for patients with fertility problems." (G. Haidl and J. P. Allam, Andrologia, Vol. 40 (1), 2008)

"This book details innovative methods for analyzing gene expression, chromosome organization, and proteins in single cells and cell extracts. ... The book is written for basic science and clinical researchers, particularly those providing care to couples seeking advice regarding infertility and inherited genetic disorders. ... The chapters are written by experts and the book is carefully edited. ... The laboratory protocols and tips will be valued by laboratory scientists interested in forensic medicine, reproductive medicine, and developmental genetics." (Bruce A. Fenderson, Doody's Review Service, October, 2007)

Spis treści

Table of Contents 1. Non-invasive metabolic assessment of single cells David K. Gardner 2. Laser Capture Microdissection for Analysis of Single Cells Nobuki Nakamura, Katharina Ruebel, Long Jin, Xiang Qian, Heyu Zhang, and Ricardo V. Lloyd 3. Fluorescence in situ hybridization (FISH) on Single Cells (Sex Determination and Chromosome Rearrangements) Paul N. Scriven and Caroline Mackie Ogilvie 4. Single cell PCR for PGD: methods, strategies and limitations Karen Sermon and Martine De Rycke 5. Real-Time Quantitative PCR Measurement of Male Fetal DNA in maternal plasma Bernhard Zimmermann, Ahmad El-Sheikhah, Kypros Nicolaides, Wolfgang Holzgreve, Sinuhe Hahn 6. Cell-Free Fetal DNA Plasma Extraction and Real Time-PCR Quantification Jill L. Maron, Kirby L. Johnson, and Diana W. Bianchi 7. LATE-PCR and allied technologies: Real-time detection strategies for rapid, reliable diagnosis from single cells Kenneth E. Pierce and Lawrence J. Wangh 8. Efficient isothermal amplification of the entire genome from single cells Karen V. Schowalter, Jolene R. Fredrickson and Alan R Thornhill 9. Comparative Genomic Hybridization on single cells Lucille Voullaire and Leeanda Wilton 10. Generation of amplified RNAs and cDNA libraries from single mammalian cells James Adjaye 11. Real-time PCR to measure gene expression in single cells Dagan Wells 12. Gender Determination and Detection of Aneuploidy in Single Cells Using DNA Array-Based Comparative Genomic Hybridisation (Array CGH) Dong Gui Hui, Xin Yuan Guan, and Nicole Hussey 13. Microarray technology for mutation analysis of low template DNA samples Chelsea Salvado and David Cram