Selected Topics in Mass Spectrometry in the Biomolecular Sciences

ISBN-13: 9780792348498 / Angielski / Twarda / 1997 / 612 str.

Richard M. Caprioli; Giovanni Sindona; Antonio Malorni

Opis

Many fundamental aspects of the methods used in mass spectrometry are presented here, with reference to recent developments. The principles and applications of electrospray, ion spray and MALDI ionization technique are included, together with optimised GC/MS interfacing systems and tools for quantitative analysis. There is also a comprehensive treatment of modern instrumentation for mass analysis and detection.

Spis treści

Preface. Part I: Methods in Mass Spectrometry. Electrospray Ionization: Theory and Application; S.J. Gaskell. Atmospheric Pressure Ionization (ESI and APCI); A. Raffaelli. Matrix-Assisted Laser Desorption- Ionization (MALDI) Mass Spectrometry: Principles and Applications; M. Karas, U. Bahr. High Sensitivity ESI and MALDI MS; T.B. Farmer, R.M. Caprioli. Optimised GS/MS Interfacing Systems and Operating Conditions; P.J. Arpino. Quantitative Analysis by Mass Spectrometry: Some Important Considerations; M.W. Duncan. Optimization of Mass Spectrometric Ionisation Efficiency Data; I.T. Ozgen, et al. Role of Internal Energy in Mass Spectrometric Fragmentation; K. Vékey. Interaction of Metal Ions and Protons with Simple Biomolecules. Structure Information from Bond Energies and Entropy Effects; B.A. Cerda, et al. A Promising Marriage Between Theory and Experiment: Density Functional Method versus Mass Spectrometry; T. Marino, et al. Part II: Instrumentation for Mass Analysis and Detection. Recent Advances in Magnet Sector Mass Spectrometry; O.V. Menirovskiy, et al. Quadrupole Mass Filters and Quadrupole Ion Traps; R.G. Cooks, et al. Time-of-Flight Mass Spectrometry; M.L. Vestal. Combined Analyzer Technologies; P. Kofel, U.P. Schlunegger. Fourier Transform Mass Spectrometry; M.L. Easterling, I.J. Amster. Part III: Application to Biomolecules. Protein Characterization by MS in Pharmaceutical Industry; D. Muller, et al. Folding Pathways of Disulphide Containing Proteins; M. Ruoppolo, et al. Integration of Chemical Modification Reactions and Mass Spectrometric Methods in Protein Characterisation; S. Foti. PSD-MALDI Analysis of Peptides; B. Spengler. Development of a Mass Spectrometric Approach for the Characterisation of Hemoglobin Adducts; P. Ferrante, et al. Reactivity of Antineoplastic Drugs with Model Peptides Studied by Advanced Mass Spectrometry Methodologies; V. Carbone, et al. Applications of Mass Spectrometry in Biochemical Studies of Nucleosides, Nucleotides and Nucleic Acids; R.P. Newton. MALDI Mass Analysis of Oligonucleotides; U. Bahr, M. Karas. DNA Adducts in Human Carcinogenesis; H. Bartsch. MALDI MS of Oligo and Polysaccharides; D. Garozzo. Mass Spectrometry of Isoeicosanoids as Novel Lipid Substances; R.C. Murphy, R.J. Waugh. The Role of Mass Spectrometry in the Characterization of Plant Metabolites from Crude Extracts; A. De Nino, et al. Environmental Applications of Mass Spectrometry: Toxaphene Analysis; F.I. Onuska, R.J. Maguire. The Determination of Polar Compounds in the Aquatic Environment; M.J.F. Suter. Some Mass Spectrometry Contributions in the Study of Cultural Heritage; P. Agozzino. Index.