Pulsed-Field Gel Electrophoresis: Protocols, Methods, and Theories

ISBN-13: 9781489943910 / Angielski / Miękka / 2013 / 481 str.

Margit Burmeister; Levy Ulanovsky

Opis

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Recenzje

...an excellent reference and experimental manual...a must for every biochemistry and molecular biology library.-Journal of the American Chemical Society

Spis treści

Electrophoretic Techniques. Field-Inversion Gel Electrophoresis. Resolving Multi-megabase DNA Molecules Using Contour-Clamped Homogeneous Electric Fields (CHEF). Separation and Size-Determination of DNA over a 10-200 kbp Range. PFGE Using Double-Inhomogeneous Fields or Orthogonal Field-Alternating Gel Electrophoresis (OFAGE). Transverse Alternating-Field Electrophoresis. Rotating Field Gel Electrophoresis (ROFE). Preparation, Manipulation, and Pulse Strategy for One-Dimensional Pulsed-Field Gel Electrophoresis (ODPFGE). Auxiliary Methods. Preparation, Restriction, and Hybridization Analysis of Mammalian Genomic DNA for Pulsed-Field Electrophoresis. Encapsulation of Cells in Agarose Beads. Preparation and Separation of Intact Chromosomes of Vertebrates by One-Dimensional Pulsed-Field Gel Electrophoresis (ODPFGE). Isolation of Plant DNA for Pulsed-Field Gel Electrophoresis. Methyltransferases as Tools to Alter the Specificity of Restriction Endonucleases. Desktop Digital Imaging: Application to Detection of Length Heterogeneity After Hyperresonant Pulsed-Field Gel Electrophoresis of Mature Bacteriophage P22 DNA. Biological Applications. Bacterial Genome Mapping by Two-Dimensional Pulsed Field Gel Electrophoresis (2D-PFGE). Protozoan Genomes: Karyotype Analysis, Chromosome Structure, and Chromosome Specific Libraries. Construction of Yeast Artificial Chromosome Libraries by Pulsed-Field Gel Strategies for Mapping Large Regions of Mammalian Genomes. Two-Dimensional DNA Electrophoresis (2D-DE) for Mammalian DNA. Pulsed-Field and Two-Dimensional Gel Electrophoresis of Long Arrays of Tandemly from Large PFGE Fragments. In-Gel Detection of DNA: Application to Study of Viral DNA Metabolism by Use of Pulsed-Field Agarose Gel Electrophoresis. Theories and Observations. Theories of Pulsed-Field Gel Electrophoresis. The Dynamics of DNA Gel Electrophoresis. Orientation of the Agarose Matrix by Pulsed Electric Fields. Reptation Theory of Pulsed Electrophoresis and Trapping Electrophoresis. Elastic Bag Model of One-Dimensional Pulsed-Field Gel Electrophoresis (ODPFGE). Index.