Proteins at Solid-Liquid Interfaces

ISBN-13: 9783642069130 / Angielski / Miękka / 2010 / 330 str.

Philippe Dejardin; Philippe D. Jardin

Opis

This book opens with a description of fundamental aspects of protein adsorption to surfaces, a phenomenon that plays a key role in biotechnological applications, especially at solid-liquid interfaces. Presented here are methods for studying adsorption kinetics and conformational changes such as optical waveguide lightmode spectroscopy (OWLS). Also described are sensitive bench techniques for measuring the orientation and structure of proteins at solid-liquid interfaces, including total internal reflection ellipsometry (TIRE), dual polarisation interferometry (DPI) and time of flight - secondary ion mass spectrometry (TOF SIMS). A model study of fibronectin at polymer surfaces is included, as are studies using microporous membranes and textiles with immobilized enzymes for large-scale applications. Biocompatibility, anti-fouling properties and surface modification to modulate the adsorption and activity of biomolecules are among the other topics addressed in this invaluable book. "

Spis treści

Analysis of the Adsorption Kinetics.- Protein Adsorption Kinetics: Influence of Substrate Electric Potential.- From Kinetics to Structure: High Resolution Molecular Microscopy.- Initial Adsorption Kinetics in a Rectangular Thin Channel, and Coverage-Dependent Structural Transition Observed by Streaming Potential.- Analysis of the Structure at the Interface.- Dual Polarisation Interferometry: An Optical Technique to Measure the Orientation and Structure of Proteins at the Solid-Liquid Interface in Real Time.- Total Internal Reflection Ellipsometry: Monitoring of Proteins on Thin Metal Films.- Conformations of Proteins Adsorbed at Liquid-Solid Interfaces.- Evaluation of Proteins on Bio-Devices.- Some Applications.- Fibronectin at Polymer Surfaces with Graduated Characteristics.- Development of Chemical Microreactors by Enzyme Immobilization onto Textiles.- Approaches to Protein Resistance on the Polyacrylonitrile-based Membrane Surface: an Overview.- Modulation of the Adsorption and Activity of Protein/Enzyme on the Polypropylene Microporous Membrane Surface by Surface Modification.- Nonbiofouling Surfaces Generated from Phosphorylcholine-Bearing Polymers.