Plant Electrophysiology: Methods and Cell Electrophysiology

ISBN-13: 9783642435928 / Angielski / Miękka / 2014 / 364 str.

Alexander G. Volkov

Opis

This book compiles new findings from the work of internationally renowned experts in plant electrophysiology, biophysics, bioelectrochemistry, ion channels, membrane transport, imaging of water transport, photosynthesis, mechanosensors, osmotic motors, sensing and actuation in plants. First volume covers modern methods in plant electrophysiology and cell electrophysiology. Second volume deals with signal transduction and responses in plants.

Spis treści

At the roots of plant neurobiology.- Plant electrostimulation and data acquisition.- Plant response to stress: Microelectrode voltage clamp studies.- Application of non-invasive microelectrode flux measurements in plant stress physiology.- Intracellular measurements of the electrical properties of walled cells.- Making contact and measuring cellular electrochemical gradients.- Studying membrane transport processes by non-invasive microelectrodes: Basic principles and methods.- Multi-electrode array – a new approach to plant electrophysiology.- Electrochemical impedance spectroscopy.- Patch clamp techniques for plant cells.- pH banding in Charophyte algae.- Membrane excitation and cytoplasmic streaming as modulators of photosynthesis and proton flows in Characean cells.- Functional characterization of plant ion channels in heterologous expression systems.- Mechanism of passive permeation of ions and molecules through plant.

Blurb

This book, written by the leading scientists in the field of plant electrophysiology, provides a comprehensive and up-to-date overview of single-cell electrophysiology and current methods in plant electrophysiology. The first part begins with a historical introduction to plant electrophysiology. Chapters describe methods of measuring the membrane potentials, ion fluxes, trans-membrane ion gradients, ion-selective microelectrode measurements, the patch-clamp technique, multi-electrode arrays, electrochemical impedance spectroscopy, data acquisition, and electrostimulation methods. The second part deals with plant cell electrophysiology, including chapters on the electrophysiology of Characean cells, functional characterization of plant ion channels, and mechanisms of passive permeation of ions and molecules through plant membranes.