Peptide Synthesis: Methods and Protocols

ISBN-13: 9781071602263 / Angielski / Twarda / 2019 / 338 str.

Waleed M. Hussein; Mariusz Skwarczynski; Istvan Toth

Spis treści

1. Solution Phase Peptide Synthesis: The Case of Biphalin

            Dagmara Tymecka and Aleksandra Misicka

 

2. Application of Fmoc-SPPS, Thiol-Maleimide Conjugation and Copper(I)-Catalyzed Alkyne-Azide Cycloaddition “Click” Reaction in the Synthesis of a Complex Peptide-Based Vaccine Candidate against Group A Streptococcus

            Chuankai Dai, Rachel J. Stephenson, Mariusz Skwarczynski, and Istvan Toth

 

3. In Situ Neutralization Protocols for Boc-SPPS

            Ramkrishna Adhikary and Philip E. Dawson

 

4. Anhydrous Hydrogen Fluoride Cleavage in Boc Solid Phase Peptide Synthesis

            Kirtikumar B. Jadhav, Katrina J. Woolcock, and Markus Muttenthaler

 

5. Automated Solid-Phase Peptide Synthesis

            Dirk F.H. Winkler

 

6. New Developments in Microwave-Assisted Solid Phase Peptide Synthesis

            Sandeep K. Singh and Jonathan M. Collins

 

7. Protecting Groups in Peptide Synthesis

            Maddeboina Krishnaiah and Martin Conda-Sheridan

 

8. Synthesis of O-Acyl Isopeptides: Stepwise and Convergent Solid-Phase Synthesis

            Taku Yoshiya

 

9. An Isodipeptide Building Block for Microwave-Assisted Solid-Phase Synthesis of Difficult Sequence-Containing Peptides

            Waleed M. Hussein, Mariusz Skwarczynski, and Istvan Toth

 

10. SPOT Synthesis: The Solid-Phase Peptide Synthesis on Planar Surfaces

            Dirk F.H. Winkler

 

11. Solid-Phase Synthesis of Wollamide Cyclohexapeptide Analogs

            Allan M. Prior and Dianqing Sun

 

12. Synthesis of Branched Peptides via a Side-Chain Benzyl Ester

            Junjie Liu, Jian Li, Xiaobo Tian, Feng Tang, and Wei Huang

 

13. Solid Phase Synthesis of Octapeptin Lipopeptides

            Karl A. Hansford, Zyta M. Ziora, Matthew A. Cooper, and Mark A.T. Blaskovich

 

14. Peptidomimetic Synthesis―Drug Discovery for Alzheimer’s Disease

            Yoshio Hamada and Zyta M. Ziora

 

15. Synthesis of Amide Backbone-Modified Peptides

            Abu-Baker M. Abdel-Aal, Richard Raz, George Papageorgiou, and John Offer

 

16. Synthesis of NickFects, a New Family of CPPs, by Solid-Phase Peptide Synthesis

            Piret Arukuusk and Ülo Langel

 

17. Chemoenzymatic Synthesis of HIV-1 Glycopeptide Antigens

            Guanghui Zong, Chao Li, and Lai-Xi Wang

 

18. Synthesis of Antimicrobial Lipopeptides Using the “CLipPA” Thiol-Ene Reaction

            Yann O. Hermant, Alan J. Cameron, Paul W.R. Harris, and Margaret A. Brimble

 

19. Decarboxylative Couplings for Late-Stage Peptide Modifications

            Meng Yao Zhang and Lara R. Malins

 

20. Peptide and Pseudopeptide Bond Synthesis in Phosphorus Dipeptide Analogs

            Artur Mucha and Paweł Kafarski

 

21. Micro-Bioreactor Techniques for the Production and Spectroscopic Characterization of Microbial Peptides

            Zeinab G. Khalil, Angela A. Salim, and Robert J. Capon

 

22. Metal Peptide Complexes for Study Neurodegenerative Diseases

            Mariusz Mital, Jakub P. Sęk, and Zyta M. Ziora

Blurb

This book provides a variety of procedures for synthetically producing peptides and their derivatives, ensuring the kind of precision that is of paramount importance for successful synthesis. Numerous techniques relevant to drugs and vaccines are explored, such as conjugation and condensation methodologies. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, Peptide Synthesis: Methods and Protocols serves as an essential guide to the many crucial processes that will allow researchers to efficiently prepare, purify, characterize, and use peptides for chemical, biochemical, and biological studies.