Microtubule Protocols

ISBN-13: 9781617376917 / Angielski / Miękka / 2010 / 305 str.

Jun Zhou

Opis

This book presents a comprehensive collection of essential and up-to-date methods for studying both the biology of microtubules and the mechanisms of action of microtubule-interacting drugs. The book contains a straightforward presentation of readily reproducible protocols, tips for troubleshooting, and advice on avoiding common mistakes. Basic scientists and clinical researchers will benefit from this collection.

Spis treści

Microtubule Protocols Editor: Zhou, Jun (Nankai University, Tianjin, China) Table of Contents: Chapter 1 (review) Microtubules: an overview Richard H. Wade Chapter 2 Large scale purification of brain tubulin with the modified Weisenberg procedure José Manuel Andreu Chapter 3 Purification and mass-spectrometry identification of microtubule-binding proteins from Xenopus egg extracts Vincent Gache, Patrice Waridel, Sylvie Luche, Andrej Shevchenko and Andrei V. Popov Chapter 4 Preparation and characterization of post-translationally modified tubulins from Artemia Franciscana Paul A. O'Connell and Thomas H. MacRae Chapter 5 Studying the structure of microtubules by electron microscopy Linda A. Amos and Keiko Hirose Chapter 6 Fluorescence microscopy of microtubules in cultured cells Irina Semenova and Vladimir Rodionov Chapter 7 (review) Measurements of stathmin-tubulin interaction in solution Marie-France Carlier Chapter 8 Analysis of microtubule dynamics by polarized light Rudolf Oldenbourg Chapter 9 Visualization of spindle behavior using confocal microscopy Adam I. Marcus Chapter 10 Live cell approaches for studying kinetochore-microtubule interactions in Drosophila Daniel W. Buster and David J. Sharp Chapter 11 Analysis of tubulin transport in nerve processes Andrey Tsvetkov and Sergey Popov Chapter 12 Analysis of microtubule-mediated intracellular viral transport Chunyong Liu, Min Liu and Jun Zhou Chapter 13 Xenopus oocyte wound healing as a model system for analysis of microtubule-actin interactions Tong Zhang and Craig A. Mandato Chapter 14 Screening for inhibitors of microtubule-associated motor proteins Frank Kozielski, Salvatore DeBonis and DimitriosSkoufias Chapter 15 (review) Clinical pharmacology and use of microtubule-targeting agents in cancer therapy Amelia B. Zelnak Chapter 16 Studying drug-tubulin interactions by X-ray crystallography Audrey Dorleans, Marcel Knossow and Benoît Gigant Chapter 17 Characterizing ligand-microtubule binding by competition methods José Fernando Díaz and Rubén Martínez Buey Chapter 18 Methods for studying vinca alkaloid interactions with tubulin Sharon Lobert and John J. Correia Chapter 19 High-throughput screening of microtubule-interacting drugs Susan L. Bane, Rudravajhala Ravindra and Anna A. Zaydman Chapter 20 (review) Strategies for the development of novel taxol-like agents Susan L. Mooberry

Blurb

Microtubules are essential components of the cytoskeleton, and play critical roles in a variety of cell processes, including cell shaping, intracellular tracking, cell division, and cell migration. Microtubule Protocols presents a comprehensive collection of essential and up-to-date methods for studying both the biology of microtubules and the mechanisms of action of microtubule-interacting drugs. The straightforward presentation of readily reproducible protocols is a hallmark of the Methods in Molecular Medicine™ series, and is evident in this volume. Methods presented range from the purification and characterization of microtubule proteins, analysis of post-translational modifications of tubulin, and determination of microtubule structure, to the visualization of microtubule and spindle behavior, measurement of microtubule dynamics, and examination of microtubule-mediated cellular processes. Both basic scientists and clinical researchers will benefit from this collection of state-of-the-art protocols for microtubule research.