Methods for Protein Analysis: A Practical Guide for Laboratory Protocols

ISBN-13: 9781475715071 / Angielski / Miękka / 2013 / 228 str.

Jr. Robert A. Copeland

Opis

As protein science continues to become an increasingly important aspect of academic and commercial sciences and technology, the need has arisen for a ready source of laboratory protocols for the analysis and evaluation of these biological polymers. Methods for Protein Analysis presents the methods most relevant to the generalist bench scientist working with proteins. A concise yet thorough summary, it covers laboratory methods that can be reasonably performed in a standard protein laboratory, without specialized equipment or expertise. Taking a how to approach, this book examines the techniques used to answer common protein analytical questions and describes methods useful in daily laboratory work. Methods for Protein Analysis is the ideal reference for protein laboratories in academic, government and industrial settings. It is an essential benchtop manual for first-year graduate students beginning their laboratory experience as well as for chemists, biochemists, and molecular biologists in the pharmaceutical, biotechnological, food and specialty chemical industries, and for analysts concerned with the purity and structural integrity of protein. Featuring illustrations and a convenient spiral binding, this guide offers a glossary of common abbreviations and a list of suppliers for protein science.

Recenzje

This book is an excellent benchtop manual for any scientist who analyses proteins - Annals of Clinical Biochemistry; This book is an excellent benchtop manual for any scientist who analyses proteins - Annals of Clinical Biochemistry; This book is an excellent benchtop manual for any scientist who analyses proteins - Annals of Clinical Biochemistry

Spis treści

Introduction to protein structure; General methods for handling proteins; Methods for protein quantitation; Electrophoretic and chromatographic methods for assessing protein purity; Immunological methods; Detection of non-protein components; Peptide mapping and amino acid analysis; Residue-specific chemical modification of proteins; Spectroscopic probes of protein structure; Protein folding and stability; Appendix I: Glossary of common abbreviations in protein science; Appendix II: Suppliers of reagents and equipment for protein science.

O autorze

Robert A. Copeland, PhD, is Department Head of Enzymology and Mechanistic Pharmacology at GlaxoSmithKline, and Adjunct Professor of Biochemistry and Biophysics at the University of Pennsylvania School of Medicine.