Isolation and Purification of Proteins

ISBN-13: 9780824707262 / Angielski / Twarda / 2003 / 676 str.

Rajni Hatti-Kaul; Bo Mattiasson; Hatti-Kaul Hatti-Kaul

Opis

This publication details the isolation of proteins from biological materials; techniques for solid-liquid separation, concentration, crystallization, chromatography, scale-up, process monitoring and product formulation; and regulatory and commercial considerations in protein production.

Spis treści

Resolution technique for protein separation, selectivity in affinity chromatography, genetic approaches to facilitate purification, solid-liquid separation, membranes for protein isolation and purification, precipitation of proteins - non-specific and specific, protein crystallization for large-scale bioseparation, extraction for rapid protein isolation, adsorption as an initial step for the capture of proteins, fast chromatography of proteins, novel approaches to the chromatography of proteins, scale up and commercial production of recombinant proteins, process monitoring in downstream processing, protein formulation, regulatory requirements, cleaning and sanitization issues in protein purification.

O autorze

Rajni Hatti-Kaul, Bo Mattiasson