Interfacial Enzyme Kinetics

ISBN-13: 9780471493044 / Angielski / Twarda / 2002 / 328 str.

Mahendra Kumar Jain; Otto G. Berg; Otto G. Berg

Opis

A vast number of biochemical reactions are catalysed by molecules fixed to the surface of membranes (or other biological structures) with molecules in the surrounding solution. The study of the mechanisms at these "Biointerfaces" are becoming increasingly important for the understanding of biological catalysts, such as enzymes. This project is the first book to deal with the physical and chemical principles of an emerging field of science, for which the authors have set the ground-work.
* The first book to deal with this newly emerging area.

* Concentrates on the chemical and physical foundation of enzyme catalysis

* Key area for the deeper understanding of biocatalytic processes

* Examples for proteins and nucleic acids, two central areas of biochemical and bioorganic research

Recenzje

"...outlines the principles, rules, and analytic protocols for the kinetic analysis of the catalytic functions of enzymes at interfaces." ( SciTech Book News, Vol. 26, No. 2, June 2002)

Spis treści

Preface
Theory Boxes
List of Symbols
Why Interfacial Enzymes?
Interface Phenomena: Accessibility and Exchange
To Be or Not To Be: Dilemma for the Substrate in Solution
Interfacial Processivity: Ensemble Behavior in the Scooting Mode
Analysis of the Processive Reaction Progress
Detailed Balance Conditions for Interfacial Equilibria
Rapid Substrate Replenishment in the Quasi–Scooting Mode
Interfacial Allostery
Inhibition: Specific or Nonspecific
The Delay to the Steady State in the Reaction Progress
Nonidealities of the Dispersed Phases
Effects of Reduction of Dimensionality

Blurb

A vast number of biochemical reactions are catalyzed by molecules fixed to the surface of membranes (or other biological structures). Although trans–membrane processes have been extensively studied for the last 30 years, the focus on interfacial processes has lagged. The study of the mechanisms at these ′biointerfaces′ is becoming increasingly important for the understanding of biological catalysts. This book is the first to deal with the physical and chemical principles of an emerging field of science, for which the authors have established the groundwork. While several specialized reviews have appeared on the various aspects of the subject, this text serves as a definitive primer; it puts this interdisciplinary field on a firm footing, develops key features of interfacial enzyme kinetics that include variables related to turnover processivity, and substrate accessibility and replenishment.

Key features:
∗ The first book in the field – concise, thorough and authoritative.
∗ The fundamentals developed in this book connect basic physical chemistry to biophysical chemistry, surface and colloid chemistry, enzymology, pharmacology, and cell biology.
∗ The key interfacial processes of turnover, processivity and replenishment are examined and it is shown how together they determine the catalytic efficiency of interfacial enzymes.
∗ Focuses on introducing a usable yet rigorous quantitative basis for analysis of interfacial catalysis in terms of the primary rate and equilibrium constants.
∗ Describes the quantitative characterization of function and inhibition of interfacial enzymes under conditions relevant for evaluation of their pharmacological potential.
In addressing issues and concerns in a growing field that borders physical, biological and surface sciences, this book will be of interest to a broad spectrum of scientists in such diverse fields as biochemistry, biophysics, enzymology, biotechnology, biomaterial science (phospholipid interfaces), pharmacology, bioorganic chemistry and colloid and interface sciences. As well as providing an informative text for advanced undergraduate and postgraduate students, many researchers in industry will also benefit from this comprehensive treatise.