Chemokines: Methods and Protocols

ISBN-13: 9781627034258 / Angielski / Twarda / 2013 / 238 str.

Astrid E. Cardona; Eroboghene E. Ubogu

Opis

Chemokines constitute a large family of structurally similar cytokines that contain a signature of conserved cysteine residues joined by disulfide bridges. Binding of chemokines to specific G protein-coupled receptors followed by downstream signaling defines their biological function. Initially, chemoattraction was the key function linked to chemokines/chemokine receptors; however, in recent years, it has become clear that chemokine ligand-receptor interactions can also modulate cellular activation, survival, and proliferation, among other functions in homeostatic and diseased states. Importantly, major advances in our understanding of chemokine biology have led to chemokine receptors becoming specific therapeutic targets with great potential. In Chemokines: Methods and Protocols, expert researchers provide practical information regarding experimental models and state of the art protocols used to delineate chemokine/chemokine receptor function and their applications in health and disease. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Practical and easy to use, Chemokines: Methods and Protocols aims to reveal key protocols of functional and descriptive chemokine ligand/receptor assays that will be of practical significance to graduate students, post-doctoral fellows, trainees, and researchers in academia and industry.

Spis treści

1. The Fine Balance of Chemokines During Disease: Trafficking, Inflammation, and Homeostasis

            Sandra M. Cardona, Jenny A. Garcia, and Astrid E. Cardona

 

2. Chemokine-Dependent Signaling Pathways in the Peripheral Nervous System

            Eroboghene E. Ubogu

 

3. Multispectral Imaging and Automated Laser Capture Microdissection of Human Cortical Neurons: A Quantitative Study of CXCR4 Expression

            Jonathan Pitcher, Roberto Wurth, Saori Shimizu, and Olimpia Meucci

 

4. Chemokine Receptors and Neural Stem Cells 

            Meizhang Li

 

5. Chemokine Receptor Interactions with Virus-Like Particles

            Luigi Buonaguro, Maria Tagliamonte, and Maria Luisa Visciano

 

6. Chemokine Receptor Antagonist Development

            Alexandre Garin, Zoë Johnson, Aurelie Hermant, Fanny Beltran, Yann Ratinaud, Alexandra Michel, Sonja Krohn, Marilène Gaudet, Susanna Carboni, Hong Ji, Marc Missotten, Olivier Leger, Christine Power, and Amanda Proudfoot

 

7. A Novel Approach to Quantify G Protein-Coupled Receptor Dimerization Equilibrium Using Bioluminescence Resonance Energy Transfer

            Irina Kufareva, Bryan Stephens, C. Taylor Gilliland, Beili Wu, Gus Fenalti, Damon Hamel, Raymond C. Stevens, Ruben Abagyan, and Tracy M. Handel

 

8. Unraveling Chemokine and Chemokine Receptor Expression Patterns Using Genetically Engineered Mice

            Simon Yona, Ki-Wook Kim, Rebecca Haffner, and Steffen Jung

 

9. Zebrafish as a Model to Study Chemokine Function

            Eva Kochhan and Arndt F. Siekmann

 

10. Inflammatory Macrophage Migration in Experimental Autoimmune Encephalomyelitis

            William J. Karpus

 

11. Visualizing Chemokine-Dependent T Cell Activation and Migration in Response to Central Nervous System Infection

            Monica J. Carson and Emma H. Wilson

 

12. Epigenetic Regulation of Chemokine/Chemokine Receptor Expression

            Anne-Marie Baird, Kenneth J. O’Byrne, and Steven G. Gray

 

13. Using Fluorescent Chemokine Uptake to Detect Chemokine Receptors by Fluorescent Activated Cell Sorting

            Laura B. Ford, Chris A.H. Hansell, and Robert J.B. Nibbs

 

14. Live Cell Imaging of Chemotactic Dendritic Cell Migration in Explanted Mouse Ear Preparations

            Michele Weber and Michael Sixt

 

15. Studying Extracellular Signaling Utilizing a Glycoproteomic Approach: Lectin Blot Surveys, a First and Important Step

            Jing Cao, Shuzhen Guo, Ken Arai, Eng H Lo, and MingMing Ning

            Sandra M. Cardona, Jenny A. Garcia, and Astrid E. Cardona

 

2. Chemokine-Dependent Signaling Pathways in the Peripheral Nervous System

            Eroboghene E. Ubogu

 

3. Multispectral Imaging and Automated Laser Capture Microdissection of Human Cortical Neurons: A Quantitative Study of CXCR4 Expression

            Jonathan Pitcher, Roberto Wurth, Saori Shimizu, and Olimpia Meucci

 

4. Chemokine Receptors and Neural Stem Cells 

            Meizhang Li

 

5. Chemokine Receptor Interactions with Virus-Like Particles

            Luigi Buonaguro, Maria Tagliamonte, and Maria Luisa Visciano

 

6. Chemokine Receptor Antagonist Development

            Alexandre Garin, Zoë Johnson, Aurelie Hermant, Fanny Beltran, Yann Ratinaud, Alexandra Michel, Sonja Krohn, Marilène Gaudet, Susanna Carboni, Hong Ji, Marc Missotten, Olivier Leger, Christine Power, and Amanda Proudfoot

 

7. A Novel Approach to Quantify G Protein-Coupled Receptor Dimerization Equilibrium Using Bioluminescence Resonance Energy Transfer

            Irina Kufareva, Bryan Stephens, C. Taylor Gilliland, Beili Wu, Gus Fenalti, Damon Hamel, Raymond C. Stevens, Ruben Abagyan, and Tracy M. Handel

 

8. Unraveling Chemokine and Chemokine Receptor Expression Patterns Using Genetically Engineered Mice

            Simon Yona, Ki-Wook Kim, Rebecca Haffner, and Steffen Jung

 

9. Zebrafish as a Model to Study Chemokine Function

            Eva Kochhan and Arndt F. Siekmann

 

10. Inflammatory Macrophage Migration in Experimental Autoimmune Encephalomyelitis

            William J. Karpus

 

11. Visualizing Chemokine-Dependent T Cell Activation and Migration in Response to Central Nervous System Infection

            Monica J. Carson and Emma H. Wilson

 

12. Epigenetic Regulation of Chemokine/Chemokine Receptor Expression

            Anne-Marie Baird, Kenneth J. O’Byrne, and Steven G. Gray

 

13. Using Fluorescent Chemokine Uptake to Detect Chemokine Receptors by Fluorescent Activated Cell Sorting

            Laura B. Ford, Chris A.H. Hansell, and Robert J.B. Nibbs

 

14. Live Cell Imaging of Chemotactic Dendritic Cell Migration in Explanted Mouse Ear Preparations

            Michele Weber and Michael Sixt

 

15. Studying Extracellular Signaling Utilizing a Glycoproteomic Approach: Lectin Blot Surveys, a First and Important Step

            Jing Cao, Shuzhen Guo, Ken Arai, Eng H Lo, and MingMing Ning