ISBN-13: 9781119886327 / Angielski / Twarda / 2023
Preface ixEditor's Biographies xiList of Contributors xiii1 EPR Spectroscopy 11.1 Outline of EPR Spectroscopy 2Hiroyuki Mino1.1.1 Overview 21.2 Biological Applications of EPR 13Isao Suetake, Risa Mutoh, Yuichi Mishima, Masatomo So, and Hironobu Hojo1.2.1 Proteins and Their Structures: Domain and Intrinsically Disordered Region 131.2.2 Introduction of Spin Probes on Proteins 141.2.3 Measurement of Constant Wave (CW)-EPR Spectrum 191.2.4 Application of CW-EPR to Protein (Clock Protein, Amyloid Proteins, and HP1) 211.2.4.1 Clock Proteins 221.2.4.2 Amyloid Proteins (Aß Peptide, ß 2 -microglobulin, alpha-synuclein, Tau, and Prion) 231.2.4.3 Heterochromatin Protein 1 (HP1) 261.2.5 Measurement of Longer Distance between Spin-spin (HP1, Tau, alpha-synuclein) 291.2.6 Biophysical Functions of Protein Dynamics 311.2.7 Summary/Conclusion 312 Introduction to Incoherent Neutron Scattering: A Powerful Technique to Investigate the Dynamics of Bio-macromolecules 39Tatsuhito Matsuo and Judith Peters2.1 Introduction 392.2 Basic Theory and Dynamical Information Obtained from iNS 422.2.1 Basic Principle of iNS Experiments 422.2.2 Incoherent Scattering Function 452.2.3 Dynamical Information Obtained by iNS 512.2.3.1 Elastic Incoherent Neutron Scattering (EINS) 522.2.3.2 Quasi-elastic Neutron Scattering (QENS) 532.3 Examples of Biological Applications of iNS 582.3.1 Dynamical Modulation of Proteins Caused by a Disease-causing Point Mutation 582.3.2 Dynamical Differences between Amyloid Polymorphic Fibrils Showing Different Levels of Cytotoxicity 592.3.3 New Theoretical Framework to Describe the Dynamical Behavior of Lipid Molecules 602.3.4 Separation of Dynamics of Protein-detergent Complexes 612.3.5 Hydration Water Mobility around Proteins 622.4 Summary 633 Elucidation of Protein Function Using Raman Spectroscopy 69Saima Malik, Maitrayee U. Trivedi, Gurpreet K. Soni, and Rohit K. Sharma3.1 Introduction 693.2 Basic Principle and Working of Raman Spectroscopy 713.2.1 Theory and Frequencies of Raman Spectroscopy 713.2.2 Instrumentation 733.3 Advances in Raman Spectroscopy Techniques 743.3.1 Resonance Raman Spectroscopy for Protein Analysis 743.3.1.1 Ultraviolet Resonance Raman Spectroscopy 753.3.1.2 Time-resolved Resonance Raman Spectroscopy 763.3.2 Surface-enhanced Raman Spectroscopy (SERS) 773.3.3 Tip-enhanced Raman Spectroscopy 803.3.4 Polarized Raman Spectroscopy 833.3.5 Raman Crystallography 853.3.6 2D-COS Raman Spectroscopy 883.4 Applications 913.5 Conclusion 924 Fundamental Principles of Impedance Spectroscopy and its Biological Applications 101Yusuke Tsutsui4.1 Introduction 1014.1.1 Basic Concept of Impedance Spectroscopy 1014.1.2 Description of Impedance for Capacitors and Inductors 1054.1.3 Nyquist Plot 1064.1.4 Debye Model 1084.1.5 Constant Phase and Warburg Element to Model Distorted and Diffusive Components 1114.2 Biological Applications of Impedance Spectroscopy 1134.2.1 Detection of DNA Hybridization and Photodamage 1134.2.2 Detection and Analysis of Proteins 1154.3 Conclusion 1195 Mass Spectrometry Imaging 125Shuichi Shimma5.1 Introduction 1255.2 Workflow of MSI 1265.3 Mass Microscope 1285.4 Visualization of Small Molecules (Pharmaceutical) 1285.5 Structural Isomer Discrimination Imaging (Steroid Hormones) 1305.6 Visualization of Proteins (Intact, Digestion) 1335.7 Visualization of Protein Function (Enzymatic Activity Visualization) 1345.8 Summary 1396 Elucidation of Protein Function Using Single-molecule Monitoring by Quantum Dots 143Maitrayee U. Trivedi, Deepika Sharma, Alisha Lalhall, Rohit K. Sharma, and Nishima Wangoo6.1 Introduction 1436.1.1 Introduction to Quantum Dots 1446.1.2 Types of Quantum Dots 1456.1.2.1 Core Type QDs 1456.1.2.2 Core/shell-type QDs 1476.1.2.3 Alloyed-type QDs 1486.2 Synthesis Methods 1486.2.1 Wet-chemical Methods 1506.2.2 Vapor-phase Methods 1506.3 Bioconjugation 1516.4 Analytical Methods for Single-molecule Monitoring by Quantum Dots 1526.4.1 Epifluorescence Microscopy 1526.4.2 Total Internal Reflection Fluorescence Microscope 1536.4.3 Confocal Microscopy 1546.4.4 pseudo-TIRFM 1546.4.5 Single-point Edge Excitation Subdiffraction Microscopy 1566.5 Applications 1566.5.1 Application of Single-molecule Monitoring Using QD for Enlightening Nanoscale Neuroscience 1566.5.2 Investigation of Diffusion Dynamics of Neuroreceptors in Cultured Neurons 1576.5.3 Single-molecule Tracking of Neuroreceptors in Intact Brain Slices (in Vivo) 1586.5.4 QD-tagged Neurotransmitter Transporters 1606.5.5 QD Labeled Serotonin Transporter (SERT) to Understand Membrane Dynamics 1606.5.6 Membrane Trafficking and Imaging of Dopamine Transporter (DAT) Using QDs 1616.6 Limitations of QDs 1636.7 Conclusion 1637 Biological Solid-state NMR Spectroscopy 169Toshimichi Fujiwara7.1 Introduction 1697.2 Magnetic Interactions for NMR 1707.2.1 Zeeman Interaction 1707.2.2 Isotropic and Anisotropic Chemical Shifts 1707.2.3 Homo- and Heteronuclear Dipolar Interactions 1717.3 Methods for Solid-state NMR 1737.3.1 Sample Preparation of Solid-state NMR 1737.3.2 Experimental NMR Techniques for High-resolution Solid-state NMR 1747.3.3 Fast MAS for ¯1 H NMR 1767.3.4 Multidimensional High-resolution NMR Experiments with Recoupling RF Pulse Sequences 1767.3.5 Paramagnetic Effects for Structural Analysis 1777.3.6 High-field DNP for Sensitivity Enhancement 1787.3.7 Oriented Molecular Systems 1797.4 Applications of Solid-state NMR to Biological Molecular Systems 1807.4.1 Membrane Proteins and Peptides 1807.4.2 Amyloid Fibrous Proteins 1827.4.3 In-situ Cellular Biomolecules 1847.5 Concluding Remarks 1848 Electrically Induced Bubble Knife and Its Applications 191Yoko Yamanishi8.1 Introduction 1918.2 Electrically Induced Bubble Knife 1928.3 Electrically Induced Bubble Injector 1998.3.1 Bubble Formation with Reagent Interface 2008.3.2 Simultaneous Injection and Ablation 2008.4 Plasma-induced Bubble Injector 2018.5 Protein Crystallization by Electrically Induced Bubbles 2028.6 Protein Crystallization by Plasma-induced Bubbles 207Index 215
Isao Suetake, Ph.D, is Professor at Nakamura Gakuen University, Japan. He has published widely on the molecular mechanisms of epigenetics.Rohit K. Sharma, Ph.D, is Assistant Professor in the Department of Chemistry, Panjab University, India. His research focuses on peptide/protein conjugated nanostructures in drug delivery and related areas.Hironobu Hojo, Ph.D, is Professor at the Institute for Protein Research, Osaka University, Japan. He has published widely on the chemical synthesis of proteins and related subjects.
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