A Handbook for Dna-Encoded Chemistry: Theory and Applications for Exploring Chemical Space and Drug Discovery

ISBN-13: 9781118487686 / Angielski / Twarda / 2014 / 480 str.

Robert A. Goodnow Jr.

Opis

This book comprehensively describes the development and practice of DNA-encoded library synthesis technology. Together, the chapters detail an approach to drug discovery that offers an attractive addition to the portfolio of existing hit generation technologies such as high-throughput screening, structure-based drug discovery and fragment-based screening. The book:

Provides a valuable guide for understanding and applying DNA-encoded combinatorial chemistry Helps chemists generate and screen novel chemical libraries of large size and quality Bridges interdisciplinary areas of DNA-encoded combinatorial chemistry - synthetic and analytical chemistry, molecular biology, informatics, and biochemistry Shows medicinal and pharmaceutical chemists how to efficiently broaden available -chemical space- for drug discovery Provides expert and up-to-date summary of reported literature for DNA-encoded and DNA-directed chemistry technology and methods

This book comprehensively describes the development and practice of DNA–encoded library synthesis technology.

Spis treści

Preface vii

Acknowledgments ix

Introductory Comments xi

Contributors xxiii

1 Just enough knowledge 1
Agnieszka Kowalczyk

2 A brief history of the development of combinatorial chemistry and the emerging need for DNA–encoded chemistry 19
Robert A. Goodnow, Jr.

3 A brief history of DNA–encoded chemistry 45
Anthony D. Keefe

4 DN A–Compa tible Chemistry 67
Kin–Chun Luk and Alexander Lee Satz

5 Foundations of a DNA–encoded library (DEL) 99
Alexander Lee Satz

6 EXERCISES IN THE SYNTHESIS OF DNA–ENCODED LIBRARIES 123
Steffen P. Creaser and Raksha A. Acharya

7 the dna tag: A Chemical gene designed for DNA–encoded libraries 153
Andrew W. Fraley

8 Analytical challenges for DNA–encoded library systems 171
George L. Perkins and G. John Langley

9 Informatics: Functionality and architecture for DNA–encoded library production and screening 201
John A. Feinberg and Zhengwei Peng

10 Theoretical considerations of the application of DNA–encoded libraries to drug discovery 213
Charles Wartchow

11 Begin with the End in Mind: The hit–to–lead process 231
John Proudfoot

12 Enumeration and Visualization of Large Combinatorial Chemical Libraries 247
Sung–Sau So

13 Screening Large Compound Collections 281
Stephen P. Hale

14 Reported applications of DNA–encoded library chemistry 319
Johannes Ottl

15 Dual–Pharmacophore DNA–Encoded Chemical Libraries 349
Jörg Scheuermann and Dario Neri

16 Hit Identification and Hit Follow–up 357
Yixin Zhang

17 Using DNA to Program Chemical Synthesis, Discover New Reactions, and Detect Ligand Binding 377
Lynn M. McGregor and David R. Liu

18 the changing feasibility and economics of chemical diversity exploration with DNA–encoded combinatorial approaches 417
Robert A. Goodnow, Jr.

19 Keeping the promise? An outlook on DNA chemical library technology 427
Samu Melkko and Johannes Ottl

Index 435

O autorze

ROBERT A. GOODNOW, JR. is the Executive Director of the Chemistry Innovation Centre, Discovery Sciences at AstraZeneca and the Founder of GoodChem Consulting, LLC. He has extensive experience in drug discovery and chemistry technologies including carbohydrate, combinatorial, oligonucleotide, and analytical chemistry. Robert Goodnow has authored or coauthored nearly 100 publications and patents and has spoken extensively on the topics of drug lead finding and the outsourcing of drug discovery chemistry. He has lectured as an adjunct professor of medicinal chemistry.

Blurb

The theory, methods, and practices needed to generate and screen very large, high–quality, small molecule, and DNA–encoded libraries

Describing the development and practice of DNA–encoded library synthesis technology, this book expertly details an approach to drug discovery that offers an attractive addition to the portfolio of existing hit generation technologies such as high–throughput screening, structure–based drug discovery, and fragment–based screening. A Handbook for DNA–Encoded Chemistry is comprised of chapters summarizing practical methods, theoretical analysis, and reported applications. Important aspects of this technology, including DNA–compatible chemistry, DNA–encoded library synthesis, design of chemical genes, analytical methods for small molecule–DNA libraries, selection methods, hit identification, and DNA–directed chemistry are explored.

This book offers practicing chemists a variety of benefits:

• Guidance for understanding and applying DNA–encoded combinatorial chemistry to generate and screen novel chemical libraries
• A bridge to the interdisciplinary areas of DNA–encoded combinatorial chemistry synthetic and analytical chemistry, molecular biology, informatics, and biochemistry
• Instruction for medicinal and pharmaceutical chemists to efficiently broaden available chemical space for drug discovery
• Expert and up–to–date summary of reported literature for DNA–encoded and DNA–directed chemistry technology and methods

Despite many publications from laboratories specializing in DNA–encoded chemistry published since 1992, there has been no technical handbook offering this level of detail.