Atomic Force Microscopy applied to biological systems

ISBN-13: 9783848493944 / Angielski / Miękka / 2012 / 216 str.

Francesco Orsini

Opis

This book describes the application of Atomic Force Microscopy (AFM) to the study of the Xenopus laevis oocyte plasma membrane. In particular, different sample preparation protocols developed and optimized to perform AFM investigation of both external and intracellular sides of the oocyte native plasma membrane are presented and discussed in details. AFM imaging allowed visualization and dimensional characterization of protein complexes observed on both sides of the oocyte plasma membrane. In addition, a new methodological approach based on the purification of oocyte plasma membrane by ultracentrifugation on a discontinuous sucrose gradient allowed to image oocyte membrane in a physiological-like environment as well as to identify a membrane protein, the human aquaporin 4, expressed in the oocytes thus opening interesting perspectives in the AFM investigation of heterologous proteins of relevant biomedical interest. Taken together, these results confirm the potential of AFM as a useful tool for the structural characterization of proteins in native eukaryotic membranes as well as its relevance for describing the organization of protein complexes in native biological membranes.

O autorze

Francesco Orsini graduated in Physics from MilanUniversity and holds a Ph.D. in Physiology. His main scientific interests are focused on the biological applications of AFM, the study of the magnetic properties of contrast agents for MRI and molecular nanomagnets by NMR relaxometry. He is coauthor of 40 papers published in peer reviewed journals.