This book describes the application of Atomic Force Microscopy (AFM) to the study of the Xenopus laevis oocyte plasma membrane. In particular, different sample preparation protocols developed and optimized to perform AFM investigation of both external and intracellular sides of the oocyte native plasma membrane are presented and discussed in details. AFM imaging allowed visualization and dimensional characterization of protein complexes observed on both sides of the oocyte plasma membrane. In addition, a new methodological approach based on the purification of oocyte plasma membrane by...

This book guides architects, landscape designers, urban planners, agronomists and society on the implementation of sustainable rooftop farming projects. While rooftop farming experiences are sprouting all over the world the need for scientific evidence on the most suitable growing solutions, policies and potential benefits emerges.