Zymography: Methods and Protocols

ISBN-13: 9781493971091 / Angielski / Twarda / 2017 / 310 str.

Jeff Wilkesman; Liliana Kurz

Opis

This detailed volume examines the latest techniques and protocols associated with zymography, the practice of detecting enzyme activity on electrophoretic gels. Sections cover subjects such as endopeptidase zymography, reverse zymography & in situ zymography, 2D zymography, as well as a variety of special cases.

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Spis treści

Part I: Introduction

 

1. Zymography Principles

            Jeff Wilkesman and Liliana Kurz

 

Part II: Endopeptidase Zymography

 

2. Serine Protease Zymography: Low-Cost, Rapid, and Highly Sensitive RAMA Casein Zymography

            Hidetaro Yasumitsu

 

3. Cysteine Protease Zymography: Brief Review

            Jeff Wilkesman

 

4. Aspartic Protease Zymography Case Study: Detection of Fungal Acid Proteases by Zymography

            Gavin Kernaghan and Michael Mayerhofer

 

5. Detection of Aspartic Proteinase Activities Using Gel Zymography

            Handunge Kumudu Irani Perera

 

6. MMP Activity Detection in Zymograms

            Péter Bencsik, Monika Bartekova, Anikó Görbe, Krisztina Kiss, János Pálóczi, Jana Radosinska, Gergő Szűcs, and Péter Ferdinandy

 

7. Characterization of Novel Collagenolytic Proteases

            Goran Mucic, Brankica Rašković, and Natalija Polović

 

8. Zymography as a Research Tool in the Study of Matrix Metalloproteinase Inhibitors

            Zongli Ren, Juanjuan Chen, and Raouf A. Khalil

 

9. Detection and Characterization of Bacterial Proteinases Using Zymography

            Madathiparambil G. Madanan and Ambili Mechoor

 

10. A Sensitive, Rapid, and Specific Technique for the Detection of Collagenase Using Zymography

            Shivcharan Prasad and Ipsita Roy

 

Part III: Reverse Zymography and In Situ Zymography

 

11. Reverse Zymography: Overview and Pitfalls

            Kanika Sharma and Debasish Bhattacharyya

 

12. Cell In Situ Zymography: Imaging Enzyme-Substrate Interactions

            Aastha Chhabra and Vibha Rani

 

Part IV: 2D Zymography

 

13. Examination of Gelatinase Isoforms in Rodent Models of Acute Neurodegenerative Diseases Using Two-Dimensional Zymography

            Shanyan Chen, Fanjun Meng, Zhenzhou Chen, Zhe Qu, Jiankun Cui, and Zezong Gu

 

14. Two-Dimensional Zymography of Proteases from Steatotic Duck Liver

            Jeff Wilkesman, María Fernanda Padrón, Liliana Kurz, and Hervé Rémignon

 

Part V: Special Zymography Cases

 

15. Simultaneous Detection of Activity and Relative Molecular Mass of Adenylate Kinases after SDS-PAGE and Blotting

            Silvia Ravera and Isabella Panfoli

 

16. Silver-Stained Fibrin Zymography: Separation of Proteases and Activity Detection Using a Single Substrate-Containing Gel

            Chang-Su Park, Dae-Ook Kang, and Nack-Shick Choi

 

17. Zymography Methods to Simultaneously Analyze Superoxide Dismutase and Catalase Activities: Novel Application for Yeast Species Identification

            Esther Gamero-Sandemetrio, Rocío Gómez-Pastor, and Emilia Matallana

 

18. Detection of Guaiacol Peroxidase on Electrophoretic Gels

            Diana Castro, Lellys M. Contreras, Liliana Kurz, and Jeff Wilkesman

 

19. In Situ Demonstration and Characteristic Analysis of the Protease Using Substrate Immersing Zymography

            He HaiLun, Li Hao, and Liu Dan

 

20. Use of Zymography in Trypanosomiasis Studies

            Jéssyka Fernanda Santiago Monte, Cláudia Jassica Gonçalves Moreno, Joana Patrícia Molato Figueiredo Lopes Monteiro, Hugo Alexandre de Oliveira Rocha, Aline Rimoldi Ribeiro, and Marcelo Sousa Silva

 

21. Zymography in Multiwells for Quality Assessment of Proteinases

            Ambili Mechoor and Madanan G. Madathiparambil

 

22. Visualization of Enzyme Activities in Earthworm Biopores by In Situ Soil Zymography

            Bahar S. Razavi, Duyen Hoang, and Yakov Kuzyakov

 

23. Multiplex Cathepsin Zymography to Detect Amounts of Active Cathepsins K, L, S, and V

            Manu O. Platt

 

24. Transfer Zymography

            Daniel Pan, Karl A. Wilson, and Anna Tan-Wilson

 

25. Sequential Detection of Thermophilic Lipase and Protease by Zymography

            Liliana Kurz, Zully Hernández, Lellys M. Contreras, and Jeff Wilkesman

 

26. Calpain Zymography: General Methodology and Protocol

            Kevin K.W. Wang

 

27. CTAB Zymography for the Analysis of Aspartic Proteases from Marine Sponges

            Oscar González and Jeff Wilkesman

 

28. Zymography Detection of a Bacterial Extracellular Thermoalkaline Esterase/Lipase Activity

            María Tapizquent, Marleny Fernández, Georgina Barreto, Zully Hernández, L. Mariela Contreras, Liliana Kurz, and Jeff Wilkesman

 

29. Amylase Zymography

            Adarelys J. Andrades Mundo and Lellys M. Contreras

Blurb

This detailed volume examines the latest techniques and protocols associated with zymography, the practice of detecting enzyme activity on electrophoretic gels. Sections cover subjects such as endopeptidase zymography, reverse zymography & in situ zymography, 2D zymography, as well as a variety of special cases. Written in the highly respected Methods in Molecular Biology series format, chapters include brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Comprehensive and practical, Zymography: Methods and Protocols serves as an ideal aid for a broad array of researchers from the fields of biological and medicinal science, as well as scientists working in clinical and diagnostic medicine, medical genetics, agricultural entomology, genetic monitoring of environmental pollution, and forensic science.