Real-Time PCR

ISBN-13: 9780415377348 / Angielski / Miękka / 2006 / 362 str.

M Tevfik Dorak

Opis

Provides the basic principles of applied real-time PCR. This work also provides a grounding for those who wish to develop further applications.

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Recenzje

The goal is to provide both theoretical and practical information for those wishing to use real time PCR as a laboratory tool. This is a generally worthwhile aim and, with some caveats, suitably met. This is a useful book in that limitations and uses are both covered. Doody's Notes, February 2007

Spis treści

1. An Introduction to Real-time PCR  2. Data Analysis and Reporting  3. Relative Quantification  4. Normalisation  5. High-Throughput Primer and Probe Design  6. Quantitative Analysis of Ocular Gene Expression  7. Quantitative Gene Expression by Real-time PCR  8. Real-time PCR Using SYBR Green  9. High-Resolution Melting Analysis for Scanning and Genotyping  10. Quantitative Analyses of DNA Methylation  11. Mitochondrial DNA Analysis  12. Real-time Immuno-PCR  13. Clinical Microbiology  14. Clinical Virology  15. Solid Organ Transplant Monitoring  16. Real-time PCR Applications in Haematology  17. Real-time PCR for Prenatal Diagnosis of Monogenic Diseases Caused by Single Nucleotide Changes - the Example of the Haemoglobinopathies

O autorze

Tevfik Dorak-Medical Department, University of Newcastle upon Tyne, Newcastle, UK