Non-Radioactive Labelling : A Practical Introduction

ISBN-13: 9780122760457 / Angielski / Miękka / 1997 / 160 str.

And Forgue Garman and Forgue

Opis

Biological Techniques is a series of volumes aimed at introducing to a wide audience the latest advances in methodology. The pitfalls and problems of new techniques are given due consideration, as are those small but vital details not always explicit in the methods sections of journal papers.
In recent years, most biological laboratories have been invaded by computers and a wealth of new DNA technology and this will be reflected in many of the titles appearing in the series.
The books will be of value to advanced researchers and graduate students seeking to learn and apply new techniques, and will be useful to teachers of advanced undergraduate courses involving practical or project work.
Labelled biomolecules are an essential tool in life science research, and non-radioactive labels are becoming increasingly important due to their convenience of measurement, greater safety and lack of disposal problems compared to radioactive labels.
This book provides practical information, background theory and protocols to allow a beginner to label many types of biomolecules, including proteins, peptides, nucleic acids and small molecules.
This book is essential for biochemists, molecular biologists and cell biologists wanting to use non-radioactively labelled molecules.

Aimed at researchers without specific expertise in chemistry, the book includes:

• A review of the main signal systems and labels available, indicating their strengths and weaknesses
• Discussion of the most useful strategies for labelling the various biomolecules
• 32 protocols covering common labelling needs
• Descriptions of the factors governing protocol design, enabling protocols to be modified for different applications
• Sources of information including references, data and suppliers

Recenzje

"No doubt, this well-written book is the ideal gift for entrants in the field wanting to learn how to have their biomolecules labeled without going through the fatiguing and time-consuming bureacratic procedures for permission to work with radioactive hazards... a very useful source of the necessary background, or protocols and of ancillary information usually not reported in detail in journals." --EUROPEAN JOURNAL OF HISTOCHEMISTRY

Spis treści

Introduction to Non-Radioactive Signals and Labels. Proteins and Peptides: Chemistry Background. Conjugation of Proteins with Enzymes and Other Proteins. Fluorescent Labelling of Proteins and Peptides. Use of Tags in the Labelling and Detection of Biomolecules. Labelling of Oligonucleotides. Labelling of Long Nucleic Acid Probes. Labelling of Small Biomolecules. Appendices.