Metalloproteins: Methods and Protocols

ISBN-13: 9781627037938 / Angielski / Twarda / 2014 / 299 str.

Juan C. Fontecilla-Camps; Yvain Nicolet

Opis

Metalloproteins are involved in many key biological processes such as gas transport and metabolism, photosynthesis, cell respiration, the Krebs cycle and many other vital redox reactions. Metalloproteins: Methods and Protocols addresses multiple aspects of metalloenzyme research from the production and purification of metalloproteins using both standard recombinant techniques and a cell-free system, to the electrochemical analysis of metalloproteins, as well as IR techniques, Mossbauer spectroscopy and the theoretical interpretation of metalloenzyme catalytic and redox processes, to name a few topics. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols and notes on troubleshooting and avoiding known pitfalls.Authoritative and easily accessible, Metalloproteins: Methods and Protocols seeks to serve both professionals and novices interested in the metalloprotein field.

Spis treści

1. Advances in Research on Metalloproteins

            Perry A. Frey

 

2. Purification of O2-Sensitive Metalloproteins

            Carlos Echavarri-Erasun, Simon Arragain, and Luis M. Rubio

 

3. Expression and Purification of NifB Protein from Aerobic and Anaerobic Sources

            Carlos Echavarri-Erasun, Simon Arragain, Alessandro A. Scandurra, and Luis M. Rubio

 

4. Techniques for the Production, Isolation and Analysis of Iron-Sulfur Proteins

            Jason C. Crack, Jeffrey Green, Andrew J. Thomson, and Nick E. Le Brun

 

5. Cell-Free Synthesis of the H-cluster: a Model for the In Vitro Assembly of Metalloprotein Metal Centers

            Jon M. Kuchenreuther, Stacey A. Shiigi, and James R. Swartz

 

6. Electrochemistry of Metalloproteins – Protein Film Electrochemistry for the Study of E. coli [NiFe]-Hydrogenase-1

            Rhiannon M. Evans and Fraser A. Armstrong

 

7. FTIR Spectroscopy of Metalloproteins

            Oscar Gutiérrez-Sanz, Olaf Rüdger, and Antonio L. De Lacey

 

8. Characterizing Millisecond Intermediates in Hemoproteins Using Rapid-Freeze-Quench Resonance Raman Spectroscopy

            Hirotoshi Matsumura and Pierre Moënne-Loccoz

 

9. A Practical Guide for Nuclear Resonance Vibrational Spectroscopy (NRVS) of Biochemical Samples and Model Compounds

            Hongxin Wang, Ercan Alp, Yoshitaka Yoda, and Stephen P. Cramer

 

10. Study of Metalloproteins Using Continuous Wave Electron Paramagnetic Resonance (EPR)

            Serge Gambarelli and Vincent Maurel

 

11. Mössbauer Spectroscopy

            Martin Clémancey, Geneviève Blondin, Jean-Marc Latour, and Ricardo Garcia-Serres

 

12. X-Ray Absorption Spectroscopy of Metalloproteins

            Jesse Ward, Emily Ollmann, Evan Maxey, and Lydia A. Finney

 

13. X-Ray Crystallographic Studies of Metalloproteins

            Anne Volbeda

 

14. Quantum Mechanical Methods for the Investigation of Metalloproteins and Related Bioinorganic Compounds

            Luca Bertini, Maurizion Bruschi, Ugo Cosentino, Claudio Greco, Giorgio Moro, Giuseppe Zampella, and Luca De Gioia

 

15. Density Functional Theory – Broken Symmetry (DFT-BS) Methodology Applied to Electronic and Magnetic Properties of Bioinorganic Prosthetic Groups

            Jean-Marie Mouesca

Blurb

Metalloproteins are involved in many key biological processes such as gas transport and metabolism, photosynthesis, cell respiration, the Krebs cycle and many other vital redox reactions. Metalloproteins: Methods and Protocols addresses multiple aspects of metalloenzyme research from the production and purification of metalloproteins using both standard recombinant techniques and a cell-free system, to the electrochemical analysis of metalloproteins, as well as IR techniques, Mössbauer spectroscopy and the theoretical interpretation of metalloenzyme catalytic and redox processes, to name a few topics. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.

Authoritative and easily accessible, Metalloproteins: Methods and Protocols seeks to serve both professionals and novices interested in the metalloprotein field.