Membrane Proteins: A Laboratory Manual

ISBN-13: 9783540107491 / Angielski / Miękka / 1981 / 258 str.

A. Azzi; U. Brodbeck; P. Zahler

Opis

The growing interest in the field of biological membranes in recent years is documented by the very large number of articles, reviews, journals and books which are appearing in this field. Why then now a manual on "Membrane Proteins"? The answer is multifold. The protocols which were distributes by the teachers and lecturers at the FEBS-SKMB Course organized in Bern appeared to be very useful not only during the Course to correctly perform the experi ments, but also for the future benefit of other students and other curses. To us they appeared very modern and of simple execution, ideal for a Uni versity Advanced Course, a Summer School, or similar scientific initiatives. The possibility was also foreseen that such a manual could be used by pro fessional scientists, although not initiated into the problems, assumptions and intricacies of biochemical methodology. There are also many research teams who study proteins, for example of human fluids, and who will certainly be interested in the application of new but simply described methods. At the same time we present the student with some more complicated physical techniques which are, however, simply described and easy to execute."

Spis treści

Analytical Techniques.- Two-Dimensional Electrophoresis of Membrane Proteins.- Analysis of Membrane Antigens by Means of Quantitative Detergent- Immunoelectrophoresis(With 3 Figures and 7 Plans).- Two-Dimensional Thin Layer Chromatography. Separation of Lipids Extracted from Native and Phospholipase A2 Treated Human Erythrocyte Ghosts (With 1 Figure).- Separation of Fatty Acids from Plant Membrane Lipids by a Combined GLC-TLC Procedure.- Isolation of Proteins.- Affinity-Chromatography of Mouse Histocompatibility Antigens on Lens culinaris Lectin-Sepharose (With 3 Figures).- Isolation of a Membrane Protein: The Phospholipase A2 from Sheep Erythrocyte Membranes.- Isolation and Purification of the Nicotinic Acetylcholine Receptor from Torpedo Electric Organ.- Preparation of Spectrin-Free Vesicles from Human Red Blood Cells (With 5 Figures).- Purification by Affinity Chromatography of Red Cell Membrane Acetylcholinesterase (With 5 Figures).- Purification of Cytochrome c Oxidase by Affinity Chromatography.- Reconstitution.- Reconstitution of Pure Acetylcholine Receptor (With 2 Figures).- Reconstitution and Assay of Mitochondrial ADP/ATP Transport with a Partially Purified ADP/ATP Carrier Protein (With 3 Figures).- Investigations on the Purple Membrane of Halobacterium halobium (With 2 Figures).- Enzymology of Succinate: Ubiquinone Reductase in Detergent Solution and Reconstituted Membranes.- Isolation and Reconstitution of Band 3, the Anion Transporter of the Human Red Blood Cell Membrane (With 3 Figures).- Modification.- Chemical Modification of a Membrane Protein by Hydrophobic and Hydrophilic Labeling Reagents (With 2 Figures).- Crosslinking of an Amphiphilic Protein: Human Erythrocyte Membrane Acetylcholinesterase (With 2 Figures).- Labeling of Membranes from Within the Lipid Core: Use of a Photo- activatable Carbene Generator (With 2 Figures).- Characterization — Spectral Techniques.- Applications of Spin-Labels to Biological Systems (With 14 Figures).- The Application of Fluorescence Spectroscopy to the Study of Biological Membranes (With 2 Figures).- Use of Potentiometric Cyanine Dye in the Study of Reconstituted Membrane Proteins (With 3 Figures).- CD Measurements to Probe the Structure of the Purple Membrane (With 5 Figures).- Characterization — Other Techniques.- Lipid-Protein Interactions in Monolayers at the Air-Water Interface (With 7 Figures).- Incorporation of the Polypeptide Hormone ACTH into Planar Lipid Bilayers: Evaluation by the Capacitance Minimization Technique (With 6 Figures).- Demonstration of Liposomes by Electron Microscopy (With 6 Figures).