Kategoria BISAC: Science >> Research & Methodology

The potential of human embryonic stem cells to advance not only regenerative medicine applications but also our fundamental understanding of stem cell biology continues to drive interest in research with these cells. This detailed volume collects some of the most interesting and useful protocols that have emerged in the area over the last several years. Written in the highly successful "Methods in Molecular Biology" series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory...

This brief goes back to basics and describes the Quantitative structure-activity/property relationships (QSARs/QSPRs) that represent predictive models derived from the application of statistical tools correlating biological activity (including therapeutic and toxic) and properties of chemicals (drugs/toxicants/environmental pollutants) with descriptors representative of molecular structure and/or properties. It explains how the sub-discipline of Cheminformatics is used for many applications such as risk assessment, toxicity prediction, property prediction and regulatory decisions apart...

This volume includes comprehensive and up-to-date coverage of all nucleic-acid-specific steps used in X-ray crystallography, from macromolecule production to structure determination. Chapters dedicated to RNA preparation and crystallogenesis will be of interest to beginners, while chapters focused on data collection, phasing and refinement will be particularly useful to researchers with a higher level of expertise. Several functional case studies are also presented in the last part of the book. Written in the highly successful "Methods in Molecular Biology "series format, chapters include...

The second edition of this volume provides novel protocols for the study of newly discovered function and modifications of components of the Hh pathways. The first few chapters discuss methods for stable delivery of soluble Hh proteins in cell cultures that can be adapted for in vivo studies, and mathematical models for studying Hh proteins gradient formation in tissues. The next few chapters explore studies in cell culture using fibroblasts that respond readily to Hh proteins. The book goes on to present protocols for evaluation of transcriptional activity of the Gli transcription factors...

A Scientific Peak...

In Digital Methods, Richard Rogers proposes a methodological outlook for social and cultural scholarly research on the Web that seeks to move Internet research beyond the study of online culture. It is not a toolkit for Internet research, or operating instructions for a software package; it deals with broader questions. How can we study social media to learn something about society rather than about social media use? Rogers proposes repurposing Web-native techniques for research into cultural change and societal conditions. We can learn to reapply such "methods of the medium" as...

Research in the College Context, 2^nd Edition provides faculty, students, practitioners, and researchers in the college environment with a manual of diverse approaches and methods for researching higher education and college students. The text offers the reader a variety of qualitative and quantitative research tools including interviewing, surveys, mixed methods, focus groups, visual methods, participatory action research, policy analysis, document analysis and historical methods, secondary data analysis, and use of large national data sets. This revised edition...

Divided into three parts this volume summarizes the most important areas of Cell-Penetrating Peptides (CPP) research . Part one briefly presents the historical background of CPP studies and the classifications of the available CPPs, and then summarizes the approaches for prediction of novel CPPs. Part two mainly describes the methods for studies of naked CPPs, that is, CPPs without conjugated cargos. Last but not least part three presents a representative and brief summary of functionality issues of CPPs, both in vitro and in vivo. As a volume in the highly successful...

This volume presents a comprehensive overview of all the existing methods on analysing protein arginylation, from the early methods utilizing crude protein preparations and whole-cell assays to the latest advanced methods involving recombinant protein techniques, antibodies, high precision mass spectrometry, and chemical probes. This book also includes essays from the founders of the field, who originally discovered arginylation in the early 1960s and brought it to international recognition. Written in the highly successful Methods in Molecular Biology series format, chapters...

Research in the College Context, 2^nd Edition provides faculty, students, practitioners, and researchers in the college environment with a manual of diverse approaches and methods for researching higher education and college students. The text offers the reader a variety of qualitative and quantitative research tools including interviewing, surveys, mixed methods, focus groups, visual methods, participatory action research, policy analysis, document analysis and historical methods, secondary data analysis, and use of large national data sets. This revised edition...

This book critically examines how mathematical modelling shapes and limits a scientific approach to the natural world and affects how society views nature. It questions concepts such as determinism, reversibility, equilibrium, and the isolated system, and challenges the view of physical reality as passive and inert. Dan Bruiger argues that if nature is real, it must transcend human representations. In particular, it can be expected to self-organize in ways that elude a mechanist treatment.

This interdisciplinary study addresses several key areas: the "crisis" in modern physics and...

This book continues the series Electroanalytical Chemistry: A Series of Advances, " "designed to provide authoritative reviews on recent developments and applications of well-established techniques in the field of electroanalytical chemistry. Electroanalytical techniques are used in a wide range of studies, including electro-organic synthesis, fuel cell studies, and radical ion formation.

Each chapter in this volume provides comprehensive coverage of a subject area, including detailed descriptions of techniques, derivations of fundamental equations, and discussions of important related...