Plant Microtechniques and Protocols

ISBN-13: 9783319199436 / Angielski / Twarda / 2015 / 576 str.

Edward C. T. Yeung; Claudio Stasolla; Michael John Sumner

Opis

A proper understanding of the structural organization of the plant body is essential to any study in plant biology. Experimental studies in vivo and in situ will lead to structural, physiological, and cellular changes of the experimental material. To study macroscopic and microscopic changes, different histological methods and microtechniques can be used as they provide valuable information of the experimental system. In addition, the observed structural changes allow investigators to set hypothesis for further studies based on one's own observation. Thus, proper selection and utilization of microtechniques are a must for the success of a research program. At present, an up-to-date collection of protocols are not readily available in the literature. The latest work in plant microtechniques was published in 1999 by Ruzin but many others are no longer in print e.g., Jensen (1964); O'Brien and McCully (1981)]. Furthermore, a majority of published works focus on techniques related to general processing and staining procedures. A comprehensive treatment that encompasses broader applications of microtechniques to other disciplines is lacking e.g., archeology, wood science, etc.]. There is a need to create a comprehensive volume of botanical methods and protocols which includes traditional and novel techniques that can be used by researchers in plant science and investigators in other disciplines that require plant microtechniques in their research and teaching. This book covers a wide variety of applications and brings them up-to-date to make them understandable and relevant, especially to students using the methods for the first time. It is our intention to create a useful reference for plant histology and related methods that will serve as a foundation for plant scholars, researchers, and teachers in the plant sciences.​

Spis treści

Contents

 

Preface

Contributors

 

Part I   Fixation, Processing, Embedding and Staining of Botanical Specimens

 

1.      A Guide to the Study of Plant Structure with Emphasis on Living Specimens

Edward C. Yeung   

 

2.      Chemical and Physical Fixation of Cells and Tissues: an Overview

Bing Q. Huang and Edward C. Yeung 

 

3.      Paraffin and Polyester Waxes

Claudio Stasolla and Edward C. Yeung

 

4.      The Glycol Methacrylate Embedding Resins – Technovit 7100 and 8100

Edward C. Yeung and Colin K. W. Chan

 

5.      Epoxy Resins for Light and Transmission Electron Microscopy

Michael J. Sumner

 

6.      LR White Acrylic Resin

Edward C. Yeung and Bing Q. Huang

 

7.      High Pressure Freezing and Freeze Substitution of In vivo and In vitro Cultured Plant Samples

Jose M. Seguí-Simarro

 

8.      Three-Dimensional Imaging for Electron Microscopy of Plastic-embedded Plant Specimens

Jose M. Seguí-Simarro

 

9.      Fluorescent Staining of Living Plant Cells

Sarah Schoor, Shiu-Cheung Lung, Dustin Sigurdson, and Simon D. X. Chuong

 

10.  Improved Methods for Clearing and Staining of Plant Samples

Alexander Lux, Marek Vaculík, and Ján Kováč

 

Part II  Microtechniques related to Cell and Molecular biology studies

 

11.  Whole Mount Immunofluorescence Staining of Plant Cells and Tissues

Masaki Shimamura                                                 

12.  Protoplast Isolation and Staining

Shiu-Cheung Lung, Sarah Schoor, Dustin Sigurdson, Makoto Yanagisawa, Kelly Yeung, Mi Qi Liu and Simon D. X. Chuong

 

13.  Guiding Principles for Live Cell Imaging of Plants using Confocal Microscopy

Subramanian Sankaranarayanan and Marcus A. Samuel

                                                                                                         

14.  Immunogold Labeling for Electron Microscopy: Strategy and Problem Solving

Fengli Guo and Bing Q. Huang

 

15.  Abscisic Acid Immunostaining

Yung-I Lee and Mei-Chu Chung

 

16.  Plant Chromosome Preparations and Staining for Light Microscopic Studies

Subhash C. Hiremath and C.C. Chinnappa

 

17.  Chromosome techniques and FISH

Mei-Chu Chung

 

18.  Detection of S-phase of Cell Division Cycle in Plant Cells and Tissues by

using 5-ethynyl-2’-deoxyuridine (EdU)

Soujanya Kuntam and Ferhan Ayaydin

 

19.  Staining Methods for Programmed Cell Death

Owen S. D. Wally and Claudio Stasolla

 

20.  Laser Microdissection of Plant Tissues

Jenna L. Millar, Michael G. Becker, and Mark F. Belmonte

                                                                                                         

21.  RNA In situ Hybridization

Shalini Mukherjee, Claudio Stasolla, and Belay Ayele

 

Part III   Preparative Methods for the Study of Wood Anatomy

 

22.  Microscopic Examination of Wood: Sample Preparation and Techniques for Light Microscopy

Jacques

C. Tardif and France Conciatori

                                                                                                             

23.  Collecting and Processing Wood Microcores for Monitoring Xylogenesis

Annie Deslauriers, Sergio Rossi, and Eryuan Liang

 

24.  Three-dimensional Imaging of Cambium and Secondary Xylem Cells by Confocal Laser Scanning Microscopy

Satoshi Nakaba, Peter Kitin, Yusuke Yamagishi, Shahanara Begum, Kayo Kudo, Widyanto Dwi Nugroho, and Ryo Funada

 

Part IV   Botanical Techniques and Protocols for Archeology and Herbarium Collection

 

25.  Collection of Plant Remains from Archaeological Contexts

Alessandra Celant, Donatella Magri, and Francesca Romana Stasolla

 

26. 

Archaeological Wood Preparation

Alessandra Celant and Gemma Coccolini

 

27.  Archaeopalynological Preparation Techniques

Donatella Magri and Federico Di Rita

 

28.  Phytoliths: Preparation and Archaeological Extraction

Brian Kooyman

 

29.  Starch Granules: Preparation and Archaeological Extraction

Brian Kooyman      

 

30.  Plant Collection, Identification, and Herbarium Procedures

Bonnie Smith and C. C. Chinnappa

 

 

Index

 

 

O autorze

Edward C. Yeung
Department of Biological Sciences, University of Calgary, Calgary, Alberta, Canada

Claudio Stasolla
Department of Plant Science, University of Manitoba, Winnipeg, Manitoba, Canada

Michael J. Sumner
Department of Biological Sciences, University of Manitoba, Winnipeg, Manitoba, Canada

Bing Q. Huang
Mayo Clinic, Rochester, Minnesota, USA

Blurb

This work gathers common plant microtechniques and updates their procedures using a simple and fully understandable approach.  The text serves as a handy resource for scientists familiar with the protocols and as a guide for the novice, especially students just beginning to learn about various structural methods for the first time.  This book analyzes a range of topics in order to generate cross-talks among scientists in different research disciplines.

The first section of this volume covers the more commonly used embedding methods, with emphasis on the preparative methods for light and electron microscopy. A number of cell biology related protocols are compiled in the second section to showcase the usefulness of various techniques based on different processing and staining methods. The third section highlights some common and recent procedures in wood preparation. The last section includes botanical methods related to archaeological uses of plant materials. A special chapter on field and herbarium procedures is also included to serve as a guide to students interested in plant collection and taxonomic studies.