Kategoria BISAC: Science >> Chemistry - Analytic

SELDI is distinct from other TOF-MS technologies in that it couples features of chromatography and mass spectrometry, facilitating analyte enrichment and sample cleanup on an array surface. In the growing field of proteomics, SELDI technology has been widely used for biomarker discovery and characterization in diverse applications including diagnostics, drug development, and basic research. SELDI-based biomarker studies can typically be divided into four phases: discovery, validation, purification and identification, and assay development. SELDI-TOF Mass Spectrometry: Methods and...

Forensic DNA profiling procedures are mainly based on high resolution and high throughput capillary electrophoresis separation and detection systems of PCR amplicons obtained from DNA genomic markers with different inheritance patterns. InDNA Electrophoresis Protocols for Forensic Genetics, expert researchers in the field detail many of the protocols and methods which are now commonly used to perform forensic DNA profiling. It includes protocols for profiling of autosomal STRs, Y-STRs, X-STRs, autosomal SNPs, INDELS, Y-SNPs, mtDNA-SNPs, and mtDNA hypervariable regions HV1 and HV2 ....

Proteins are the functional units of the cellular machinery and they provide significant information regarding the molecular basis of health and disease. Therefore, techniques to separate and isolate the various proteins are critical to studying and understanding their functional characteristics. One of the widely used techniques for this purpose is electrophoresis. In Protein Electrophoresis: Methods and Protocols, contributions from experts in the field have been collected in order to provide practical guidelines to this complex study. Each chapter outlines a specific...

Fluorescent proteins are intimately connected to research in the life sciences. Tagging of gene products with fluorescent proteins has revolutionized all areas of biosciences, ranging from fundamental biochemistry to clinical oncology, to environmental research. The discovery of the Green Fluorescent Protein, its first, seminal application and the ingenious development of a broad palette of fluorescence proteins of other colours, was consequently recognised with the Nobel Prize for Chemistry in 2008.

Fluorescent Proteins I is devoted to the basic photophysical and...

Chemical additives are used to enhance the properties of many industrial products. Since their release into the environment is a potential risk for man and nature, their fate and behavior were investigated in the framework of the European Union-funded project RISKCYCLE. The results are presented in two volumes, Global Risk-Based Management of Chemical Additives I: Production, Usage and Environmental Occurrence and Global Risk-Based Management of Chemical Additives II: Risk-Based Assessment and Management Strategies. This book is the first of the two volumes and contains two...

Glycosylation is the most abundant post-translational modification of proteins. Estimates vary widely, but a common assessment is that upwards of 50% of eukaryotic proteins are modified by some type of glycan. In Mass Spectrometry of Glycoproteins: Methods and Protocols, expert researchers in the field detail many of the methods that are now commonly used for glycoproteomics. These methods and techniques include robust sample preparation techniques; advanced chromatographic strategies for improving dynamic range; state-of-the-art mass spectrometry instrumentation and associated...

Despite achievements in the application of enzymes, antibodies and biological receptors to diagnostics and sensing, the last two decades have also witnessed the emergence of a number of alternative technologies based on synthetic chemistry. This volume shows how synthetic receptors can be designed with characteristics that make them attractive alternatives to biological molecules in the sensory and diagnostics fields, with contributions from leading experts in the area. Subjects covered include synthetic receptors for a range of biomolecules, the use of antimicrobial peptides for the...