DNA Electrophoresis Protocols for Forensic Genetics

ISBN-13: 9781617794605 / Angielski / Twarda / 2011 / 394 str.

Antonio Alonso

Opis

Forensic DNA profiling procedures are mainly based on high resolution and high throughput capillary electrophoresis separation and detection systems of PCR amplicons obtained from DNA genomic markers with different inheritance patterns. InDNA Electrophoresis Protocols for Forensic Genetics, expert researchers in the field detail many of the protocols and methods which are now commonly used to perform forensic DNA profiling. It includes protocols for profiling of autosomal STRs, Y-STRs, X-STRs, autosomal SNPs, INDELS, Y-SNPs, mtDNA-SNPs, and mtDNA hypervariable regions HV1 and HV2 . Protocols for molecular identification of non-human species and mRNA profiling for body fluid identification are also included. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

Recenzje

From the reviews:

"The purpose is to offer a comprehensive book on the most modern current electrophoretic protocols and interpretation guidelines used in forensic genetics for the analysis of amplified human DNA fragments and DNA sequencing. ... researchers, forensic experts, and others interested in the field of DNA electrophoresis protocols will find this book of immense help. ... This is a unique resource that students, researchers, and forensic experts will find both interesting and helpful." (Omer Iqbal, Doody's Book Reviews, May, 2012)

Spis treści

Part I: Current Protocols on Capillary Electrophoresis of Amplified DNA Fragments  

 

1.         An Overview of  DNA Typing Methods for Human Identification: Past, Present, and Future

Robyn Thompson, Silvia Zoppis, and Bruce McCord

 

2.         Capillary Electrophoresis and 5-channel LIF Detection of a 26plex Autosomal STR Assay for Human Identification

Carolyn R. Hill.

 

3.         Capillary Electrophoresis of Mini-STR Markers to Genotype Highly Degraded DNA Samples

Michael Coble

 

4.         Interpretation Guidelines of a Standard Y-chromosome STR 17-plex PCR-CE Assay for Crime Casework

Lutz Roewer and Maria Geppert

 

5.         Capillary Electrophoresis of an X-Chromosome STR Decaplex for Kinship Deficiency Cases

Leonor Gusmão, Cíntia Alves, Iva Gomes, and Paula Sanchéz-Diz

 

6.         A 48-plex autosomal SNP GenPlex Assay for Human Individualization and Relationship Testing

Carmen Tomas, Claus Børsting, and Niels Morling.

 

7.         Typing of 49 Autosomal SNPs by Single Base Extention and Capillary Electrophoresis for Forensic Genetic Testing

Claus Børsting, Carmen Tomas, and Niels Morling

 

8.         A 34-plex Autosomal SNP Single Base Extension Assay for Ancestry Investigations

Christopher Phillips, Manuel Fondevila, and Maviki Lareu

 

9.         SNaPshot® Minisequencing Analysis of Multiple Ancestry-informative Y-SNPs    Using Capillary Electrophoresis

Maria Geppert and Lutz Roewer

 

10.       Capillary Electrophoresis of 38 Non-coding Biallelic Mini-Indels for Degraded Samples and as Complementary Tool in Paternity Testing

Rui Pereira and Leonor Gusmão

 

11.       Capillary Electrophoresis of an 11-plex mtDNA Coding Region SNP Single Base Extension Assay for Discrimination of the most Common Caucasian HV1/HV2 Mitotype

Peter M. Vallone

 

12.       Capillary Electrophoresis of a Multiplex Reverse Transcription-Polymerase Chain Reaction to Target Messenger RNA Markers for Body Fluid Identification

Cordula Haas, Erin Hanson, and  Jack Ballantyne

 

13.       DNA Extraction from Aged Skeletal Samples for STR Typing by Capillary Electrophoresis

Rene Huel, Sylvain Amory, Ana Bilic, Stojko Vidovic, Edin Jasaragić, and Thomas Parsons

 

14.       Interpretation Guidelines for Multilocus STR Forensic Profiles from Low Template DNA Samples

Zoran M. Budimlija and Theresa Caragine

 

15.       Interpretation Guidelines of Mixed STR Multilocus Electrophoretic Profiles

Juan Antonio Luque 

 

16.       Capillary Electrophoresis Analysis of a 9-plex STR Assay for Canine Genotyping

Barbara van Asch and Antonio Amorim

 

17.       Capillary Electrophoresis of DNA from Cannabis sativa for Correlation of Samples to Geographic Origin

Heather Miller Coyle

 

18.       Capillary Electrophoresis of Multigene Barcoding Chloroplast Markers for Species Identification of Botanical Trace Evidence

Gianmarco Ferri, Beatrice Corradini, and Milena Alu

 

 

Part II: Capillary electrophoresis and DNA Sequencing

 

19.       Capillary Electrophoresis of Big-Dye Terminator Sequencing Reactions for Human mtDNA Control Region Haplotyping in the Identification of Human Remains

Marta Montesino & Lourdes Prieto

 

20.       Capillary Electrophoresis of Human mtDNA Control Region Sequences from Highly Degraded Samples using Short mtDNA Amplicons

Odile Loreille and  Jodi A. Irwin

 

21.       INTERPRETATION GUIDELINES OF MTDNA CONTROL REGION SEQUENCE ELECTROPHEROGRAMS IN FORENSIC GENETICS

Manuel Crespillo

 

22.       Capillary Electrophoresis of mtDNA Cytochrome b Gene Sequences for Animal Species Identification

Adrian Linacre

 

23.       Sequence Analysis of the Canine Mitochondrial DNA Control Region from Shed Hair Samples in Criminal Investigations

Cordula  Berger, B. Berger and Walther Parson

 

Part III: Advances in Microchip Capillary Electrophoresis

 

24.       Integrated Sample Cleanup and Microchip Capillary Array Electrophoresis for High-Performance Forensic STR Profiling

Peng Liu, Susan A. Greenspoon, Stephanie H. I. Yeung, and Richard A. Mathies

 

25.       Microchip Capillary Electrophoresis Protocol to Evaluate Quality and Quantity of mtDNA Amplified Fragments for DNA Sequencing in Forensic Genetics

Coro Fernandez and  Antonio Alonso.

 

26.       Microchip Capillary Electrophoresis of Multi Locus VNTR Analysis for Genotyping of Bacillus Anthracis and Yersinia Pestis in Microbial Forensic Cases

Andrea Ciammaruconi