Kategoria BISAC: Science >> Biotechnology

Animal Cell Biotechnology: Methods and Protocols, Second Edition constitutes a comprehensive manual of state-of-the-art and new techniques for setting up mammalian cell lines for production of biopharmaceuticals, and for optimizing critical parameters for cell culture considering the whole cascade from lab to final production. The volume is divided into five parts that reflect the processes required for different stages of production. In Part I, basic techniques for establishment of production cell lines are addressed, especially transduction techniques, cells for gene therapy and antibody...

From probe design to applications in clinical settings, this book provides a diverse set of instructive examples, guided by experts in the field who offer easy-to-follow experimentals. The book first offers an introduction to the basic principles of fluorescence and then describes applications of fluorogenic probes in real-time PCR, which currently is the gold standard for quantitative DNA and RNA analysis.

Coverage extends the potential of realtime as well as advocates simplifications of the probe technologies. It also presents a new simplified molecular beacon design,...

This book provides a comprehensive volume that integrates a wide spectrum of methods required to implement current and future progress in tissue engineering. Leading experts in various disciplines provide examples of recent advances in tissue engineering-related methodologies. Authors present authoritative discussions on topics. The book supplies an understanding of diverse technologies and methods used to drive tissue engineering into a clinical reality. The knowledge contained in this volume represents the impressive progress made in the decade when tissue engineering rapidly...

As regenerative medicine involves replacing diseased cells, tissues or organs, or repairing tissues in vivo, the manipulation of stem cells underlie its goals. In Stem Cells in Regenerative Medicine: Methods and Protocols, leading experts in the field provide an updated representation of the landscape of stem cell-based therapies in a wide spectrum of tissue systems and ontogenic stages, from the isolation and culture of stem cells to their actual use in vivo. Written in the highly successful Methods in Molecular Biology series format, these chapters include brief introductions to the...

At the time of the lightbulb's invention, not even the most imaginative thinkers could have predicted the prevention and treatment of disease through genes delivered by electric fields. In Electroporation Protocols: Preclinicaland Clinical Gene Medicine, top researchers provide hands-on protocols describing the process of non-viral delivery of genetic materials such as DNA, oligo-DNA, and RNA into targeted immune cells, tissues, and model animals in order to better treat disease. Following the successful Methods in Molecular Biology(TM) series format, each method and protocol...

NanoBioTechnology: BioInspired Devices and Materials of the Future is a groundbreaking text that will assist scientists and students in learning the fundamentals and cutting-edge nature of this new and emerging science. Focusing on materials and building blocks for nanotechnology, leading scientists from around the world share their knowledge and expertise in this authoritative volume.

The volume is broken into five sections. The first section presents an overview of nanotechnology and describes the many aspects of the field. Section 2 details biological materials serving as...

Key topics covered include a full introduction to centrifugation, sedimentation and separation; detailed coverage of centrifuge types, including batch and semi-batch centrifuges, disk-stack and tubular decanter centrifuges; methods for increasing solids concentration; laboratory and pilot testing of centrifuges; selection and sizing...

Gene transfer to animal cells was first achieved more than thirty years ago. Since then, transformation technology has developed rapidly, resulting in a multitude of techniques for cell transformation and the creation of transgenic animals. As with any expanding technology, it becomes difficult to keep track of all the developments and to find a concise and comprehensive source of information that explains all the underlying principles.

Gene Transfer to Animals Cells addresses this problem by describing the principles behind gene transfer technologies, how gene expression...