YAC Protocols

ISBN-13: 9781588296122 / Angielski / Twarda / 2006 / 204 str.

Alasdair MacKenzie

Opis

This completely revised and updated edition of YAC Protocols takes advantage of the many new advances in biology since the publication of the first edition, including the sequencing of the human genome, the genomes of a wide variety of other organisms, and the increased use of transgenic animals for understanding the molecular basis of human and animal disease. This volume also provides a much-needed update on the new techniques currently employed and to help redefine and illustrate the important roles still to be played by yeast artificial chromosome (YAC) technologies in the postgenomic age. YAC Protocols, Second Edition, demonstrates the enormous potential use of YAC technologies in understanding the role of genetics in maintaining health, promoting pathogenesis, and conferring susceptibility to disease. Offering solutions to the many problems that have plagued researchers, this new edition allows for the wider and more efficient use of YAC technologies for the analysis of genetic and pathogenic causes of human disease.

Spis treści

Markers, Selection, and Media in Yeast Artificial Chromosome Cloning David Markie Construction of Yeast Artificial Chromosome Libraries From Pathogens and Nonmodel Organisms Cecilia P. Sanchez and Michael Lanzer Analysis and Screening of Yeast Artificial Chromosome Libraries by Filter Hybridization Cecilia P. Sanchez and Michael Lanzer Analysis of Yeast Artificial Chromosome DNA by Restriction Digestion, Southern Blotting Nucleic Acid Hybridization, and Polymerase Chain Reaction Sylvia Vasiliou and John Peter Quinn Postgenomic Bioinformatic Analysis of Yeast Artificial Chromosome Sequence Kerry Miller, Scott Davidson, and Alasdair MacKenzie Transformation of Yeast Using Bioactive Beads With Surface-Immobilized Yeast Artificial Chromosomes Shigeki Kawakami, Satoshi Harashima, Akio Kobayashi, and Kiichi Fukui Modification and Amplification of Yeast Artificial Chromosomes Sanbing Shen, Anthony Harmar, and Nick Hastie Modifying Yeast Artificial Chromosomes to Generate Cre/LoxP and FLP/FRT Site-Specific Deletions and Inversions Gabriela G. Loots Selective Isolation of Large Chromosomal Regions by Transformation-Associated Recombination Cloning for Structural and Functional Analysis of Mammalian Genomes Natalay Kouprina, Vladimir N. Noskov, and Vladimir Larionov A Polymerase Chain Reaction-Mediated Yeast Artificial Chromosome-Splitting Technology for Generating Targeted Yeast Artificial Chromosomes Subclones Yeon-Hee Kim, Minetaka Sugiyama, Yoshinobu Kaneko, Kiichi Fukui, Akio Kobayashi, and Satoshi Harashima Genomic Reconstruction by Serial Mitotic Recombination of Yeast Artificial Chromosomes David Markie, Emma Jones, and Jiannis Ragoussis Targeting Vector Construction by Yeast Artificial Chromosome Modification Peter J. Murray Production of Yeast Artificial Chromosome Transgenic Mice by Pronuclear Injection of One-Cell Embryos Alasdair MacKenzie Generation of Yeast Artificial Chromosome Transgenic Mice by Intracytoplasmic Sperm Injection Pedro N. Moreira, Julio Pozueta, Patricia Giraldo, Alfonso Gutiérrez-Adán, and Lluís Montoliu Establishment of Cell Lines That Exhibit Correct Ontogenic Stage-Specific Gene Expression Profiles From Tissues of Yeast Artificial Chromosome Transgenic Mice Using Chemically Induced Growth Signals C. Anthony Blau and Kenneth R. Peterson Application of Yeast Artificial Chromosomes in Fluorescence In Situ Hybridization Thomas Liehr Contribution of Yeast Artificial Chromosome-Based Physical Maps to the Final Assembly of the Trypanosoma cruzi Genome Márcia R. M. Santos, Roberto R. M. Barros, Patrício E. Porcile, Mariano J. Levin, and José Franco da Silveira Index