This bookoffers low-cost and rapid molecular assays for the characterization of mutant plant germplasm. Detailed protocols are provided for the desiccation of plant tissues; the extraction of high-quality DNA for downstream applications; the extraction of single-strand-specific nucleases for single nucleotide polymorphism; and small insertion/deletion discovery using standard agarose gel electrophoresis. The methods described can be applied in any laboratory equipped for basic molecular biology and do away with the need for expensive freezers and toxic organic compounds. With the...
This bookoffers low-cost and rapid molecular assays for the characterization of mutant plant germplasm. Detailed protocols are provided for the des...
Souleymane Bado Brian P. Forster Joanna Jankowicz-Cieslak
This book offers effective, low-cost and user-friendly protocols for the pre-field selection of salt-tolerant mutants in cereal crops. It presents simple methods for measuring soil salinity, including soil sampling and the analysis of water-soluble salts, and describes a detailed, but simple, screening test for salt tolerance in rice, wheat and barley seedlings, which uses hydroponics. The protocols are devised for use by plant breeders and can be easily accommodated into breeding practice.
This book offers effective, low-cost and user-friendly protocols for the pre-field selection of salt-tolerant mutants in cereal crops. It presents ...
This book is open access under a CC BY-NC 2.5 license.
This book offers 19 detailed protocols on the use of induced mutations in crop breeding and functional genomics studies, which cover topics including chemical and physical mutagenesis, phenotypic screening methods, traditional TILLING and TILLING by sequencing, doubled haploidy, targeted genome editing, and low-cost methods for the molecular characterization of mutant plants that are suitable for laboratories in developing countries. The collection of protocols equips users with the techniques they need in order to start a...
This book is open access under a CC BY-NC 2.5 license.
This book offers 19 detailed protocols on the use of induced mutations in crop breedin...
This bookoffers low-cost and rapid molecular assays for the characterization of mutant plant germplasm. Detailed protocols are provided for the desiccation of plant tissues; the extraction of high-quality DNA for downstream applications; the extraction of single-strand-specific nucleases for single nucleotide polymorphism; and small insertion/deletion discovery using standard agarose gel electrophoresis. The methods described can be applied in any laboratory equipped for basic molecular biology and do away with the need for expensive freezers and toxic organic compounds. With the...
This bookoffers low-cost and rapid molecular assays for the characterization of mutant plant germplasm. Detailed protocols are provided for the des...
This open access book is one of the most important cash crops and beverages globally. Coffea arabica L. (Arabica) accounts for about 60% of the world's coffee production. Coffee leaf rust (CLR), caused by the fungus Hemileia vastatrix, is the major disease affecting Arabica coffee resulting in losses of over $1 billion annually. The geographical distribution of CLR is expanding due to climate change. Moreover, the genetic improvement of Arabica coffee is constrained due to its very narrow genetic base. This protocol book introduces essential concepts of mutation breeding as an efficient tool...
This open access book is one of the most important cash crops and beverages globally. Coffea arabica L. (Arabica) accounts for about 60% of the world'...
Ivan L. W. Ingelbrecht Maria D Joanna Jankowicz-Cieslak
This open access book is one of the most important cash crops and beverages globally. Coffea arabica L. (Arabica) accounts for about 60% of the world's coffee production. Coffee leaf rust (CLR), caused by the fungus Hemileia vastatrix, is the major disease affecting Arabica coffee resulting in losses of over $1 billion annually. The geographical distribution of CLR is expanding due to climate change. Moreover, the genetic improvement of Arabica coffee is constrained due to its very narrow genetic base. This protocol book introduces essential concepts of mutation breeding as an efficient tool...
This open access book is one of the most important cash crops and beverages globally. Coffea arabica L. (Arabica) accounts for about 60% of the world'...
