Aldose 1-epimerase or mutarotase is the enzyme that responsible for carbohydrate metabolism and converts the alpha anomer into beta anomer of glucose. This enzyme was extracted from bovine kidney cortex. Crude enzyme exhibited the activity of 14.92 U mL-1 with specific activity of 0.153 U mg-1 proteins. The enzyme activity and specific activity was increased to 53.75 UmL-1 4.981 Umg-1 respectively after 38-60% ammonium sulfate precipitation and it was further increased to 73.27 UmL-1 and 11.67 Umg-1 when subjected to diethylaminoethyl (DEAE) cellulose chromatography. Further purification was...
Aldose 1-epimerase or mutarotase is the enzyme that responsible for carbohydrate metabolism and converts the alpha anomer into beta anomer of glucose....
Isolation of active ingredients from medicinal plants and characterization can be traced to the beginning of 19th century. Large number of drugs from medicinal plants were discovered and introduced in modern pharmacopoeias during 1850-1950. In the present study, Leaves of 7 species of different medicinal plants named Hygrophila auriculata (Talmakhana) Abru precatorius (Kaincha), Morinaga olifera (Sohanjna), Withania sominifera (Ashwaganda), Cartoon tiglium (Jamal ghota), Solanum nigram (Peelo) and Psoralae corylfolia (Babachi) were put on extraction using extraction buffer. Protein contents...
Isolation of active ingredients from medicinal plants and characterization can be traced to the beginning of 19th century. Large number of drugs from ...
In this study hyper-production of citric acid Aspergillus niger was carried out using agro-industrial residues as carrier substrates in solid state fermentation (SSF) of sucrose molasses medium. It was concluded from this study that banana stalk was a good carrier substrate among the six substrates (Corncobs, corn stover, wheat straw, wheat bran and sugarcane bagasse) used for citric acid production in SSF of molasses medium and SSF gave higher citric acid production as compared to liquid state fermentation (LSF). Mutants of Aspergillus niger were produced through UV irradiations, Ethyle...
In this study hyper-production of citric acid Aspergillus niger was carried out using agro-industrial residues as carrier substrates in solid state fe...
Plants are natural source of medicinally important compounds. This study was projected to investigate the Antilipidemic activity of withania somnifera. An investigation was carried out to study the effect of Gemmo and native phytoconstituents on blood cholesterol, HDL, LDL and Triglycerides level in rabbits. Different phytoconstituents like Alkaloides, Flavonoides, Glycosides, Tannic acid, Saponins were extracted. Antilipedimic activity was determined in rabbits. Change in body weight and various parameters like serum HDL, LDL, Triglycerides and Cholesterol were determined. In feeding trials...
Plants are natural source of medicinally important compounds. This study was projected to investigate the Antilipidemic activity of withania somnifera...
Uricase is an important enzyme in the metabolism of purines catalyzing the oxidation of uric acid in the presence of oxygen, producing allantion, H2O2 and CO2. In humans and great apes, uric acid is the final product of the purine catabolism pathway - lower organisms express uricase which further degrades uric acid, however due to nonsense mutations in the urate oxidase gene (UOX pseudogene) it is not expressed in humans. Uricase enzyme was isolated from bovine kidney. The activity of crud uricase noted was3721.04 U/mL and 135.681 U/mg specific activity with 27.426 mg/mL of protein contents....
Uricase is an important enzyme in the metabolism of purines catalyzing the oxidation of uric acid in the presence of oxygen, producing allantion, H2O2...
