The popularly grown wheat is allohexaploid (2n=6X=42), consisting of three closely related sub-genomes, A, B and D. The polyploid inheritance, large genome size (~17Gb) and significant amount (~95%) of repetitive DNA render wheat recalcitrant to forward and reverse genetics approaches. In order to better understand the composition and organization of wheat genome, techniques of Cot fractionation and methylation-filtration were used. For this purpose, three different genomic DNA libraries (one each from methylation-filtered, high Cot and reassociated DNA fractions) were prepared, and random...