Biolistic DNA Delivery in Plants: Methods and Protocols

ISBN-13: 9781071603581 / Angielski / Miękka / 2021 / 334 str.

Sachin Rustgi; Hong Luo

Spis treści

Part I: Tribute to Professor Diter Von Wettstein

1. Diter von Wettstein, Professor of Genetics and Master of Translating Science into Applications

Sachin Rustgi and Birgitte Skadhauge

 

2. Diter von Wettstein and The Meiotic Program of Pairing and Recombination

Denise Zickler

 

Part II: Background and Overview

3. A Short History and Perspectives on Plant Genetic Transformation

Thakku R. Ramkumar, Sangram K. Lenka, Sagar S. Arya, and Kailash C. Bansal

 

4. Choice of The Promoter for Tissue and Developmental Stage-specific Gene

Expression

Olga G. Smirnova and Alex V. Kochetov

 

5. Choice of Explant for Plant Genetic Transformation

Nibedita Chakraborty, Priyanka Chakraborty, Moutushi Sen, and Rajib Bandopadhyay

 

6. Biolistic Approach for Transient Gene Expression Studies in Plants

Benoît Lacroix, Vitaly Citovsky

 

7. ­Nano-biolistics: An Emerging Genetic Transformation Approach

Francis J. Cunningham, Gozde S. Demirer, Natalie S. Goh, Huan Zhang, and Markita P. Landry

 

Part II: Protocols

 

8. Biolistic Transformation of Japonica Rice Varieties

Raviraj Banakar and Kan Wang

 

9. Biolistic DNA Delivery in Maize Immature Embryos

Kan Wang, Huilan Zhu, and Morgan McCaw

 

10. Biolistic DNA Delivery and its Applications in Sorghum bicolor

Guoquan Liu, Karen Massel, Basam Tabet, and Ian D. Godwin

 

11. Biolistic-mediated Gene Delivery in Sugarcane

Priya A. Joyce and Yue Sun

 

12. Genetic Transformation of Common Wheat (Triticum aestivum L.) Using Biolistics

Caroline A. Sparks, Angela Doherty

 

13. Biolistic DNA Delivery in Turfgrass Embryonic Callus Initiated from Mature Seeds

Man Zhou, Junming Zhao, Dayong Li, Shuangrong Yuan, Ning Yuan, Zhigang Li, Haiyan Jia, Fangyuan Gao, Bekir San, Qian Hu, and Hong Luo

 

14. Use of Microspore-derived Calli as Explants for Biolistic Transformation of Common Wheat

Sachin Rustgi, Samneet Kashyap, Nii Ankrah, and Diter von Wettstein

 

15. Plant Transformation Techniques: Agrobacterium- and Microparticle-mediated Gene Transfer in Cereal Plants

Jafargholi Imani and Karl-Heinz Kogel

 

16. Proteolistics: A Protein Delivery Method

Susana Martin-Ortigosa and Kan Wang

 

17. Biolistic Delivery of Programmable Nuclease (CRISPR/Cas9) in Bread Wheat

Abhishek Bhandawat, Vinita Sharma, Vikas Rishi, and Joy Roy

Blurb

This volume details protocols for the use of the biolistic DNA delivery method in different plant species. Chapters guide readers through non-protocol chapters that cover relevant topics of interest, a broad overview of the field, exciting modifications of the system, and reliable plant transformation procedures in different plant species.  Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Biolistic DNA Delivery: Methods and Protocols aims to provide a comprehensive collection of protocols to intended to be a practical guide for the novice as well as the advanced user in the field of plant genetic transformation.