Gene Silencing by RNA Interference: Technology and Application provides background in the field and describes methods and protocols for gene silencing. It offers a general introduction to the subject of gene silencing and RNA interference and focuses on technical details of the various methods of producing siRNAs and other RNAi tools. The final chapters of the book detail the application of RNAi to dissect gene function in a number of biological systems, including cell-free systems, cultured cells, and whole organisms. This book is a comprehensive guide to gene silencing by RNA interference...

Grewia tenax and Ziziphus spina-christi are two important fruit tree species; that grow wild in arid tropical regions of Pakistan and posses vital position among rural community for their multipurpose use. Ecologically, these species are well adapted to local conditions but continuous increase in aridity associated with soil salinity is a potential threat to these valuable assets of rural population. The experimental results presented in this book indicated that seeds of Ziziphus spina-christi posses physical dormancy (impermeable seed coat), which can be easily overcome through mechanical or...