Gene Silencing by RNA Interference : Technology and Application

ISBN-13: 9780849321412 / Angielski / Twarda / 2004 / 400 str.

Laurie Kelly; Muhammad Sohail; Sohail Sohail

Opis

Gene Silencing by RNA Interference: Technology and Application provides background in the field and describes methods and protocols for gene silencing. It offers a general introduction to the subject of gene silencing and RNA interference and focuses on technical details of the various methods of producing siRNAs and other RNAi tools. The final chapters of the book detail the application of RNAi to dissect gene function in a number of biological systems, including cell-free systems, cultured cells, and whole organisms. This book is a comprehensive guide to gene silencing by RNA interference methods for beginners and experienced professionals in the field.

Spis treści

Gene silencing by RNA interference and the role of small interfering RNAs; N.J. Caplen, National Institutes of Health, Maryland, USA
Basics of siRNA design and chemical synthesis; Q. Boese, A. Reynolds, and W.S. Marshall, Dharmacon, Inc. Colorado, USA
Oligonucleotide scanning arrays in the design of small interfering RNAs; V.M. Macaulay and M. Sohail, Weatherall Institute of Molecular Medicine and University of Oxford, England, UK
siRNA production by in vitro transcription; O. Donzé, P. Dudek, and D. Picard, Université de Genève and Apotech Corporation, Switzerland
Production of siRNAs with the application of deoxyribozymes; G. Doran and M. Sohail, University of Oxford, England, UK
Production of siRNA in vitro by enzymatic digestion of double-stranded RNA; F. Buchholz, D. Drechsel, M. Ruer, and R. Kittler, Max Planck Institute, Dresden, Germany
Plasmid-mediated intracellular expression of siRNAs; R. Wadhwa, M. Sano, M. Miyagishi, and K. Taira, National Institute of Advanced Industrial Science and Technology, Tsukuba, and University of Tokyo, Japan
Lentiviral vector-mediated delivery of si/shRNA, D.S. An, X-F. Qin, Vincent Auyeung, D. Baltimore, and I.S.Y. Chen, University of California-Los Angeles, UT MD Anderson Cancer Center in Texas, and California Institute of Technology, Pasadena, USA
Exogenous siRNA delivery: protocols for optimizing delivery to cells; A.J. Hollins, S.P. Fox, and S. Akhtar, Cardiff University, Wales, UK
RNAi in Drosophila cell cultures, M. Bettencourt-Dias, R. Sinka, L. Frenz, and D. Glover, University of Cambridge. and Cyclacel, Ltd., England, UK
RNAi in Caenorhabditis elegans; F. Simmer and R.H.A. Plasterk, Center for Biomedical Genetics, Utrecht, The Netherlands
Delivery of RNAi reagents in C. elegans by microinjection; P. Appleford and A. Woollard, University of Oxford, England, UK
RNAi in Drosophila embryos; R.W. Williams, University of California, Berkeley, USA
RNAi in Xenopus laevis oocytes; E. Gordon and G.W. Abbott, Cornell University, New York, USA
RNAi in Xenopus embryos; K.H. Kok and D-Y. Jin, University of Hong Kong, People's Republic of China
An RNAi-based genomic library for forward genetics in the African Trypanosome; J.C. Morris, Z. Wang, S.A. Motyka, M.E. Drew, and P.T. Englund, Clemson University, South Carolina, Massachusetts Institute of Technology, Massachusetts, Johns Hopkins University, Maryland, and Washington University, Missouri, USA
RNAi in the malaria parasite Plasmodium; A. Brüning-Richardson and G.A. McConkey, University of Leeds, England, UK
Silencing the expression of cysteine proteases (falcipains) in Plasmodium falciparum using RNA interference approaches; P.V.N. Dasaradhi, A. Mohmmed, M.J. Hossain, V.S. Chauhan, and P. Malhotra, International Center for Genetic Engineering and Biotechnology, New Delhi, India
RNA in transgenic animal models; H. Hasuwa & M. Okabe, Osaka University, Japan
RNA interference in mouse model, E. Song, P. Stern, D. Palliser, Luk Van Parijs and J. Lieberman, Harvard University, Massachusetts, USA
Virus-induced RNA silencing in plants; M. Figlerowicz, M. Barciszewska-Pacak, B. Grabowska, J. Wojciechowicz, and A. Pacak, Adam Mickiewicz University, Poznan, University of Warmia and Mazury, Olsztyn, and Polish Academy of Sciences, Poznan, Poland
RNA silencing in plants - biolistic delivery of RNAi reagents; U. Klahre and F. Meins, Novartis Research Foundation, Friedrich Miescher Institute for Biomedical Research, Basel, Switzerland
Agro-infiltration: a versatile tool for RNAi studies in plants; D. Silhavy, Agricultural Biotechnology Center, Godollo, Hungary

O autorze

Muhammad Sohail