Gradient elution demystified

Of the various ways in which chromatography is applied today, few have been as misunderstood as the technique of gradient elution, which presents many challenges compared to isocratic separation. When properly explained, however, gradient elution can be less difficult to understand and much easier to use than often assumed.

Written by two well-known authorities in liquid chromatography, High-Performance Gradient Elution: The Practical Application of the Linear-Solvent-Strength Model takes the mystery out of the practice of gradient...

Over the last 15 years, high-performance liquid chromatogra phy (LC) has made the transition from an instrument used only by experts in research labs to a tool used for routine applications by relatively unskilled workers. With this transition have come in instrumentation and column technology. In major advances the past, the operator had to be a jack-of-all-trades, with a screw driver, soldering iron, and various wrenches as constant compan ions in the LC lab. Today, many instruments contain micropro cessors as powerful as those of mainframe computers of earlier days. With this technology...

Over the last 15 years, high-performance liquid chromatogra- phy (LC) has made the transition from an instrument used only by experts in research labs to a tool used for routine applications by relatively unskilled workers. With this transition have come in instrumentation and column technology. In major advances the past, the operator had to be a jack-of-all-trades, with a screw- driver, soldering iron, and various wrenches as constant compan- ions in the LC lab. Today, many instruments contain micropro- cessors as powerful as those of mainframe computers of earlier days. With this...