The human brain represents about 2% of the body weight, yet it accounts for approximately 20% of aerobic metabolism. This high dependency on energy-consuming processes is mainly caused by the active transport of ions, which is necessary to compensate for the transmembrane ion currents that are part ofthe complex signaling processes in the brain. Ninety-five percent ofthe brain's ATP is derived from mitochondrial oxidative phosphorylation. Since that organ' s storage capacity for oxygen is minimal, any interruption of oxygen delivery to brain cells willlead to changes in membrane excitability...
The human brain represents about 2% of the body weight, yet it accounts for approximately 20% of aerobic metabolism. This high dependency on energy-co...
Leading neuroscience researchers offer a fresh perspective on neuronal function by examining all its many components-including their pertubation during major disease states-and relate each element to neuronal demands. Topics range from the dependency of neurons on metabolic supply, as well as on both ion and transmitter homeostasis, to their close interaction with the myelin sheath. Also addressed are the astrocytic signaling system that controls synaptic transmission, the extracellular matrix and space as communication systems, the role of blood flow regulation in neuronal demand and in...
Leading neuroscience researchers offer a fresh perspective on neuronal function by examining all its many components-including their pertubation durin...
There is a perception in the scientific community that the discipline of Physiology is in crisis, or at least, in a phase of profound transition and change. At the root of the problem is confusion between objectives (the biological questions to be solved) and the methods and technologies to be applied. Traditionally, ever since Claude Bernard s concept of the milieu interieur, Physiology was an integrative science with the prime concern of studying regulatory mechanisms leading to adaptation and homeostasis in the presence of challenges from a dynamic internal and external environment. This...
There is a perception in the scientific community that the discipline of Physiology is in crisis, or at least, in a phase of profound transition and c...
E. Neher and B. Sakman were the first to monitor the opening and closing of single ion channels and membranes by conductance measurements. In 1976, they used firepolished micropipets with a tip diameter of 3-5 pm to record currents from a small patch of the membranbe of sk- etal muscles, thereby decreasing background membrane noise. In order to reduce the dominant source of background noise-the leakage shunt under the pipet rim between m- the muscle membrane had to be treated brane and gla- enzymatically. Despite these early limitations, a new te- nique was born -the patch-clamp technique....
E. Neher and B. Sakman were the first to monitor the opening and closing of single ion channels and membranes by conductance measurements. In 1976, th...
Most cells will survive removal from the natural mic- environment of their in vivo tissue and placement into a sterile culture dish under optimal conditions. Not only do they survive, but they also multiply and express differen- ated properties in such a culture dish. A few cells do this in suspension, but most will need some kind of mechanical support substituting for their natural connections with other cells. The surface of a culture dish that might have to be coated is usually sufficient. The recent trend to standa- ization of conditions and the existence of commercial ent- prises with...
Most cells will survive removal from the natural mic- environment of their in vivo tissue and placement into a sterile culture dish under optimal cond...
Mit der Einfuhrung der "New Dimension"-Losungen der SAP wurden Anwendern neue Moglichkeiten eroffnet, um wichtige interne und zwischenbetriebliche Geschaftsaktivitaten ihrer Unternehmen mit einer integrierten Standardanwendungssoftware abzudecken. Neue Organisationskonzepte wie CRM, SCM oder internetbasierte Zusammenarbeit wurden mit softwaretechnischen Innovationen verknupft und ermoglichten eine umfangreichere Integration von Geschaftsprozessen. Diese neuen Themenbereiche werden vorgestellt und auf Integrationsprozesse und -beziehungen zu bestehenden R/3-ERP-Systemen untersucht. Daruber...
Mit der Einfuhrung der "New Dimension"-Losungen der SAP wurden Anwendern neue Moglichkeiten eroffnet, um wichtige interne und zwischenbetriebliche ...
Most cells will survive removal from the natural mic- environment of their in vivo tissue and placement into a sterile culture dish under optimal conditions. Not only do they survive, but they also multiply and express differen- ated properties in such a culture dish. A few cells do this in suspension, but most will need some kind of mechanical support substituting for their natural connections with other cells. The surface of a culture dish that might have to be coated is usually sufficient. The recent trend to standa- ization of conditions and the existence of commercial ent- prises with...
Most cells will survive removal from the natural mic- environment of their in vivo tissue and placement into a sterile culture dish under optimal cond...
Continuing the research of the best-selling first edition, Patch-Clamp Analysis: Advanced Techniques, Second Edition collects three more years of research in the ever-expanding study of the cell membrane. With the latest developments in the traditional patch techniques such as whole-cell and single channel as well as perforated patch, fast drug application, loose patch and macropatch techniques, this authoritative second edition updates the standard techniques while introducing three brand new, cutting-edge technical advances in the field.
Thorough and timely, Patch-Clamp Analysis:...
Continuing the research of the best-selling first edition, Patch-Clamp Analysis: Advanced Techniques, Second Edition collects three more years of r...
There is a perception in the scientific community that the discipline of Physiology is in crisis, or at least, in a phase of profound transition and change. At the root of the problem is confusion between objectives (the biological questions to be solved) and the methods and technologies to be applied. Traditionally, ever since Claude Bernard s concept of the milieu interieur, Physiology was an integrative science with the prime concern of studying regulatory mechanisms leading to adaptation and homeostasis in the presence of challenges from a dynamic internal and external environment. This...
There is a perception in the scientific community that the discipline of Physiology is in crisis, or at least, in a phase of profound transition and c...
Leading neuroscience researchers offer a fresh perspective on neuronal function by examining all its many components-including their pertubation during major disease states-and relate each element to neuronal demands. Topics range from the dependency of neurons on metabolic supply, as well as on both ion and transmitter homeostasis, to their close interaction with the myelin sheath. Also addressed are the astrocytic signaling system that controls synaptic transmission, the extracellular matrix and space as communication systems, the role of blood flow regulation in neuronal demand and in...
Leading neuroscience researchers offer a fresh perspective on neuronal function by examining all its many components-including their pertubation durin...
