to the Animal Models Volumes This volume describes animal models of drug addiction. Because of increasing public concern over the ethical treatment of animals in research, we felt it incumbent upon us to include this general preface in order to indicate why we think further research using animals is necessary. Animals should only be used when suitable alternatives are not available, and humans can only be experimented upon in severely proscribed circumstances. Alternative procedures using cell or tissue culture are inadequate in any models requiring assessments of behavioral change or of...

Despite the success of earlier Neuromethads volumes, I was initially reluctant to edit a further volume because my own - search is concerned with nonneural tissues. I changed my mind for two simple reasons. First, though the sheer diversity of ext- cellular signal molecules is staggering, still more impressive is the remarkably small number of transmembrane signaling processes they recruit- their receptors either have integral ion channels or enzyme activities, or else they catalytically activate G proteins. Likewise, when we look to the final intracellular t- gets of these signaling...

Techniques in the neurosciences are evolving rapidly. There are currently very few volumes dedicated to the methodology - ployed by neuroscientists, and those that are available often seem either out of date or limited in scope. This series is about the methods most widely used by modern-day neuroscientists and is written by their colleagues who are practicing experts. Volume 1 will be useful to all neuroscientists since it concerns those procedures used routmely across the widest range of s- disciplines. Collecting these general techniques together in a single volume strikes us not only as a...

The mammalian central nervous system depends almost - clusively on glucose as its major energy source. In addition, g- cose participates in other cerebral metabolic functions including the biosynthesis of neurotransmitters, such as acetylcholine and the amino acids. This volume of Neuromethods assembles currently available methods for the study of cerebral glucose and energy metabolism in vitro and in mm. In the first chapter, Lust et al. describe the various methods available for the appropriate fixation of brain tissue necessary for the study of cerebral energy metabolism. Different...

In this volume, we hope to cover the malor techniques that are presently being used to analyze the actions of drugs used in psyc- atry. The contributors of the chapters are active researchers who have considerable practical experience with the techniques they are describing, and the emphasis in the chapters is on three types of psychiatric drugs, namely antidepressants, antipsychotics, and anxiolytics. The first chapter, by Curry and Yu, discusses protein binding of psychotropic drugs, with special reference to equilibrium ana- sis as the method of assessment and to lipophilicity...

Recent years have seen remarkable advances in the devel- ment of techniques that have direct applications in neurological research. In consequence, the circulatory and metabolic status of the brain can be measured and correlated with changes m structure often noninvasively, m the same - and integrated function, perimental subIect This has stimulated an increased awareness of the complexity, under normal and pathological conditions, of the interdependence of these factors. Through the application of the methods described in this volume, however, these complexities can now be analyzed. The...

The development of neurophysiology, the study of the activity of living nervous tissue, has relied heavily on the techniques of electrophysiology. This emphasis is revealed in volumes 14 and 15 of this series, which show how electrophysiological techniques can be applied to research topics ranging from ion channels to human behavior. Kitai and Park show how cellular neurophysiology can be related to classical neuroanatomy, an important basis for any type of functional analysis. Wonderlin, French, Arispe, and Jones describe new (single channel) and more traditional (whole cell) techniques for...

The development of neurophysiology, the study of the activity of living nervous tissue, has relied heavily on the techniques of electrophysiology. This emphasis is revealed in volumes 14 and 15 of this series, which show how electrophysiological techniques can be applied to research topics ranging from ion channels to human behavior. Kitai and Park show how cellular neurophysiology can be related to classical neuroanatomy, an important basis for any type of functional analysis. Wonderlin, French, Arispe, and Jones describe new (single channel) and more traditional (whole cell) techniques for...

Techniques in the neurosciences are evolving rapidly. There are currently very few volumes dedicated to the methodology - ployed by neuroscientists, and those that are available often seem either out of date or limited in scope. This series IS about the methods most widely used by modern-day neuroscientists and is written by their colleagues who are practicing experts. Volume 1 will be useful to all neuroscientists since it concerns those procedures used routmely across the widest range of s- disciplines. Collecting these general techniques together in a s- gle volume strikes us not only as a...

Techniques u-r the neurosciences are evolving rapidly. There are currently very few volumes dedicated to the methodology - ployed by neuroscrentists, and those that are available often seem either out of date or limited in scope This series is about the methods most widely used by modern-day neuroscrentrsts and 1s written by their colleagues who are practicing experts Volume 1 will be useful to all neuroscientists since it concerns those procedures used routinely across the widest range of subdiscrplines Collectmg these general techmques together in a single volume strikes us not only as a...