This book was first published in 1985. For those working in molecular biology, this book describes techniques in plant genetic research and the practical application of genetic engineering to important crop plants such as the potato. The various chapters detail methods used for the genetic modification of plants, including protoplast fusion and the use of Agrobacterium and viruses as vectors for plant genes. The types of agricultural and industrial processes that will be improved by these technologies are indicated throughout the book. The contributors to this volume have prepared a...

In this third edition the authors have written a guide to the essentials of plant tissue culture. The book takes the reader through a graded series of experimental protocols and provides an introductory review of each topic. There are discussions of aseptic techniques, and nutritional components of media. Subsequent chapters cover such topics as callus formation, organogenesis, xylem cell differentiation, root cultures, cell suspensions, micropropagation by buds, somatic embryogenesis, protoplast culture and fusion, secondary metabolite production, and quantitation of procedures. A glossary,...

This book was first published in 1985. For those working in molecular biology, this book describes techniques in plant genetic research and the practical application of genetic engineering to important crop plants such as the potato. The various chapters detail methods used for the genetic modification of plants, including protoplast fusion and the use of Agrobacterium and viruses as vectors for plant genes. The types of agricultural and industrial processes that will be improved by these technologies are indicated throughout the book. The contributors to this volume have prepared a...

1 John H. Dodds The culture offragmen ts of plant tissue is not a particularly new science, in fact as long ago as 1893 Rechinger (1893) described the formation of callus on isolated fragments of stems and roots. The culture of plant tissues in vitro on a nutrient medium was performed by Haberlandt (1902), however, his attempts were unsuccessful because he chose too simple a medium that lacked critical growth factors. Over the last fifty years there has been a surge of development in plant tissue culture techniques and a host of techniques are now avail- able (Dodds and Roberts, 1982). The...