Experiments in Plant Tissue Culture

ISBN-13: 9780521478922 / Angielski / Miękka / 1995 / 276 str.

John H. Dodds; Lorin W. Roberts; J. Heslop-Harrison

Opis

In this third edition the authors have written a guide to the essentials of plant tissue culture. The book takes the reader through a graded series of experimental protocols and provides an introductory review of each topic. There are discussions of aseptic techniques, and nutritional components of media. Subsequent chapters cover such topics as callus formation, organogenesis, xylem cell differentiation, root cultures, cell suspensions, micropropagation by buds, somatic embryogenesis, protoplast culture and fusion, secondary metabolite production, and quantitation of procedures. A glossary, commercial sources of supplies, and the formulations of culture media are also included. This new edition has been completely revised and updated. The organization has been improved, and new illustrations have been added, together with new experiments on such topics as potato callus formation, and embryo culture. This book will be useful to graduate students and professionals in plant science, forestry, agronomy, and horticulture.

Recenzje

'… a worthy text and an excellent addition to any plant science laboratory.' John Love, British Society for Developmental Biology Newsletter

Spis treści

Part I. Background Information: 1. Culture of plant cells, tissues, and organs; 2. Laboratory facilities; 3. Aseptic techniques; 4. Media composition and preparation; Part II. Experimental: Callus and Callus-Derived Systems: 5. Initiation and maintenance of callus; 6. Organogenesis; 7. Cell suspensions; 8. Somatic embryogenesis; Part III. Experimental: Culture of Organs and Organized Systems: 9. Isolated roots; 10. Micropropagation by bud proliferation; 11. Anther and pollen cultures; Part IV. Experimental: Isolated Cells: 12. Transdifferentiation of parenchyma cells to tracheary elements; 13. Isolation and culture of protoplasts; 14. Protoplast fusion and somatic hybridization; Part V. Supplementary Topics: 15. Cryopreservation of germplasm; 16. Production of secondary metabolites; 17. Quantitation of procedures; 18. Formulations of tissue culture media; 19. Commercial sources of supplies; Author index; Subject index.