During their lifetime, especially when growing and dividing, cells go through various steps of the cell cycle. Knowledge of the individual steps of the cell cycle will help us understand the development of a variety of diseases better, including cancer, and also to design new drugs against it. New techniques for studying the molecular basis of these processes have recently been developed and are described in detail in this manual.
A glossary helps the reader to cope with the complex cell cycle terminology.

New techniques and updated protocols for the detection and analysis of biomolecules - proteins, glycoproteins and nucleic acids - are presented in the second edition of this successful laboratory manual. Highly sensitive systems which are widely used in molecular biological and biomedical laboratories, such as colorimetric, luminescence, fluorescence measuring using antibody/antigen binding or hybridisation, as well as PCR amplification are described in detail. The clearly structured step-by-step protocols with practical hints and a troubleshooting guide are complemented by chapters on the...

Flow cytometric analysis of molecular, biochemical, genetic and developmental parameters using cellular fluorescence techniques as well as fluorescence-activated (FACS) or magnetic (MACS) cell sorting technologies provide unique options for molecular and cellular biology. In recent years, these technologies have been considerably advanced.
In this second edition, all chapters have been updated according to the recent improvements and modifications. Further, new protocols have been added, such as on magnetic selection, magnetofluorescent liposomes, the cytometry of secreted products and...

This laboratory manual gives a thorough introduction to basic techniques. It is the result of practical experience, with each protocol having been used extensively in undergraduate courses or tested in the authors laboratory. In addition to detailed protocols and practical notes, each technique includes an overview of its general importance, the time and expense involved in its application and a description of the theoretical mechanisms of each step. This enables users to design their own modifications or to adapt the method to different systems. Surzycki has been holding undergraduate...

The book provides a review and synthesis of boreal mire ecosystems including peat soil properties, mire hydrology, carbon and nutrient cycling, and classification of mire sites. The emphasis, however, is on peatland forests as a renewable natural resource.
The approach originated in northern Europe, because there, especially in Finland, operational scale forest drainage has a long tradition based on research aiming to maintain and increase wood production on peatlands. Whenever relevant, a closer look is also given to other countries in Europe, Canada, and the USA. The results of recent...

Most information on yeasts derives from experiments with the conventional yeasts Saccaromyces cerevisiae and Schizossaccharomyces pombe, the complete nuclear and mitochondrial genome of which has also been sequenced. For all other non-conventional yeasts, investigations are in progress and the rapid development of molecular techniques has allowed an insight also into a variety of non-conventional yeasts.
In this bench manual, over 70 practical protocols using 15 different non-conventional yeast species and in addition several protocols of general use are described in...

This manual gives descriptions of the technical procedures for the establishment of primary invertebrate cell cultures in vitro, covering nutritional requirements, culture media and species-specific methods for both cell and organ cultures, as well as techniques for studies on cultured cells.