Invertebrate Tissue Culture Methods

ISBN-13: 9784431703136 / Angielski / Miękka / 2002 / 466 str.

Jun Mitsuhashi

Opis

This manual gives descriptions of the technical procedures for the establishment of primary invertebrate cell cultures in vitro, covering nutritional requirements, culture media and species-specific methods for both cell and organ cultures, as well as techniques for studies on cultured cells.

Spis treści

Preface Introduction Part I. General Methods Chapter 1. Facilities and Equipment Chapter 2. Basic Information and Overview Chapter 3. Preparation of Media Chapter 4. General Cell Culture Methods Part II. Methods for Setting Up Primary Cultures Specific to Animal Groups Chapter 5. Insecta (Lepidoptera) Chapter 6. Insecta (Diptera) Chapter 7. Insecta (Coleoptera) Chapter 8. Insecta (Hemiptera) Chapter 9. Insecta (Hymenoptera) Chapter 10. Insecta (Blattaria) Chapter 11. Insecta (Orthoptera) Chapter 12. Arthropoda Other than Insecta Chapter 13. Prochordata Chapter 14. Echinodermata Chapter 15. Mollusca Chapter 16. Annelida Chapter 17. Nematoda Chapter 18. Plathelminthes Chapter 19. Coelenterata Chapter 20. Porifera Part III. Organ cultures Chapter 21. Insecta Chapter 22. Arthropoda other than Insecta Chapter 23. Prochordata Chapter 24. Echinodermata Chapter 25. Mollusca Chapter 26. Annelida Chapter 27. Nematoda Chapter 28. Plathelminthes Chapter 29. Coelenterata Chapter 30. Porifera Part IV. Related Techniques Chapter 30. Cell Cloning Chapter 32. Karyotype Analyses Chapter 33. Identification of Cell Lines Chapter 34. Viability of Cells Chapter 35. Viable Cell Enumeration Chapter 36. Growth Rate Chapter 37. Microscope Photography Chapter 38. Virus Inoculation-Plaque Assay Chapter 39. Examination of Drug Effects Chapter 40. Cell Fusion Chapter 41. Gene technology Chapter 42. Large Scale Cell Cultures I. Composition of Salt Solutions and Culture Meda A. Salt Solutions B. Culture Media II. List of Invertebrate Continuous Cell Lines Reported III. Commercial Products of Media and List of Suppliers A. Commercial Products of Media B. List of Suppliers Index.