This book presents a comprehensive collection of essential and up-to-date methods for studying both the biology of microtubules and the mechanisms of action of microtubule-interacting drugs. The book contains a straightforward presentation of readily reproducible protocols, tips for troubleshooting, and advice on avoiding common mistakes. Basic scientists and clinical researchers will benefit from this collection.
This book presents a comprehensive collection of essential and up-to-date methods for studying both the biology of microtubules and the mechanisms ...
Microchip methods of analysis are now an essential tool for biomedical researchers. Here, the authors focus on DNA microarrays, protein microarrays, and labs-on-chips, and support the introduction of new diagnostic methods based on microtechnologies.
Microchip methods of analysis are now an essential tool for biomedical researchers. Here, the authors focus on DNA microarrays, protein microarrays...
When I was ?rst approached by Dr. Hengli Tang about writing the foreword to this book, the second edition of Hepatitis C Virus, I was a little puzzled: Book forewords are penned by persons far more famous than I am. Surely there were better people to write this, with a handful of the true visionaries in the ?eld immediately coming to my mind. However, after some thought and self-re?ection, I realized that I might just be the right person for the job. I remember the publication of the ?rst edition of Hepatitis C Virus in 1998, the year I began reading about hepatitis C virus with great...
When I was ?rst approached by Dr. Hengli Tang about writing the foreword to this book, the second edition of Hepatitis C Virus, I was a little puzzled...
Macromolecular drugs hold the promise of becoming new therapeutics for several major disorders, including cancer and cardiovascular disease. This incredible potential is explored in Macromolecular Drug Delivery, a volume which gives a wide-ranging overview of contemporary methods used in the field, and which addresses the limitations presented by a lack of safe and efficient drug delivery strategies. Chapters offer information on both in vitro and in vivo methods of macromolecular delivery, thus appealing to a broad scientific audience. Composed in the highly successful Methods in...
Macromolecular drugs hold the promise of becoming new therapeutics for several major disorders, including cancer and cardiovascular disease. This i...
In light of the critical contributions of macrophages and dendritic cells to diverse inflammatory diseases and to immunity and host defense, state-of-the-art approaches to the investigation of their behavior are essential. In Macrophages and Dendritic Cells: Methods and Protocols, expert researchers contribute laboratory protocols involving these two vital cell types functioning at the junction of the innate and acquired immune systems. The volume delves first into isolation and cell culturing then continues with topics such as phagocytosis, genetic manipulation, macrophage activation, and...
In light of the critical contributions of macrophages and dendritic cells to diverse inflammatory diseases and to immunity and host defense, state-of-...
Non-clinical drug safety evaluation, the assessment of the safety profile of therapeutic agents through the conduct of laboratory studies in in vitro systems and in animals, is an essential step in the progress of new pharmaceuticals heading toward the ultimate goal of clinical trials and, eventually, approval. In Drug Safety Evaluation: Methods and Protocols, expert researchers detail a compendium of analytical technologies with a focus on clarity and applicability in real life laboratory practice. These meticulous contributions feature key topics such as acute to chronic general toxicity...
Non-clinical drug safety evaluation, the assessment of the safety profile of therapeutic agents through the conduct of laboratory studies in in vitro ...
Efforts to describe and model the molecular structure of biological membranes go back to the beginning of the last century. In 1917, Langmuir described membranes as a layer of lipids one molecule thick 1]. Eight years later, Gorter and Grendel concluded from their studies that "the phospholipid molecules that formed the cell membrane were arranged in two layers to form a lipid bilayer" 2]. Danielli and Robertson proposed, in 1935, a model in which the bilayer of lipids is sequestered between two monolayers of unfolded proteins 3], and the currently still accepted fuid mosaic model was...
Efforts to describe and model the molecular structure of biological membranes go back to the beginning of the last century. In 1917, Langmuir describe...
Proteases decisively contribute to cancer development and promotion by regulating the activities of growth factors/cytokines and signaling receptors, as well as the composition of the extracellular matrix, thereby suppressing cell death pathways and activating cell survival pathways. In Proteases and Cancer: Methods and Protocols, expert researchers bring together a wide range of current, complimentary techniques that have been developed for the specific detection and analysis of proteases and their activities in cancer biology. Authoritative and easy to use, Proteases and Cancer: Methods and...
Proteases decisively contribute to cancer development and promotion by regulating the activities of growth factors/cytokines and signaling receptors, ...
The polymerase chain reaction (PCR) is one of the most important molecular biological methods ever devised, with numerous applications to cli- cal molecular medicine. Since its description in 1985, PCR has undergone tremendous improvements, and many variations on the basic PCR theme have been published. With such a large volume of PCR-related literature, a clinical scientist wishing to use the technique will have a difficult task loc- ing the relevant information to implement it effectively. There is thus clearly a need for an up-to-date volume with detailed protocols to facilitate the...
The polymerase chain reaction (PCR) is one of the most important molecular biological methods ever devised, with numerous applications to cli- cal mol...
Recent advances in molecular and cellular biology techniques have significantly improved our ability to detect, monitor, model and study the underlying molecular basis and pathogenesis of leukemia, yet we are still in an early discovery stage and much more work is needed in order to develop better strategies to diagnose, classify and treat this biologically and clinically diverse disease. In Leukemia: Methods and Protocols, expert researchers bring together a wide range of state-of-the-art laboratory methods and detailed protocols that are useful for both clinical and basic research...
Recent advances in molecular and cellular biology techniques have significantly improved our ability to detect, monitor, model and study the underlyin...
