Leukemia: Methods and Protocols

ISBN-13: 9781588299895 / Angielski / Twarda / 2009 / 434 str.

Chi Wai Eric So

Opis

Recent advances in molecular and cellular biology techniques have significantly improved our ability to detect, monitor, model and study the underlying molecular basis and pathogenesis of leukemia, yet we are still in an early discovery stage and much more work is needed in order to develop better strategies to diagnose, classify and treat this biologically and clinically diverse disease. In Leukemia: Methods and Protocols, expert researchers bring together a wide range of state-of-the-art laboratory methods and detailed protocols that are useful for both clinical and basic research scientists working on the disease. The volume provides techniques for prenatal backtracking of leukemic clone, molecular diagnosis, detection of genome-wide genetic abnormalities and profiling, identification of unknown fusion genes, monitoring of minimal residual diseases, disease modeling using murine and human primary hematopoietic cells, studying of normal and malignant hematopoiesis, identification of interacting partners with leukemia associated oncoproteins, and global characterization of genome-wide epigenetic changes in leukemic cells. Written in the highly successful Methods in Molecular BiologyT series format, the convenient chapters contain brief introductions, lists of the necessary materials, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Comprehensive and cutting-edge, Leukemia: Methods and Protocols will help researchers to advance knowledge and have a better understanding of the disease, which will ultimately facilitate development of anti-cancer therapy and improve quality of life for patients.

Spis treści

1 An Overview: From Discovery of Candidate Mutations to Disease Modelling and Transformation Mechanisms of Acute Leukemia Chi Wai Eric So 2 Backtracking of Leukemic Clones to Birth Joseph Wiemels, Michelle Kang, and Mel Greaves 3 Fluorescence in situ Hybridization (FISH) as a Tool for the Detection of Significant Chromosomal Abnormalities in Childhood Leukaemia Zoë J. Konn, Sarah L. Wright, Kerry E. Barber, and Christine J. Harrison 4 Specialized Fluorescence in situ Hybridization (FISH) Techniques for Leukemia Research Lyndal Kearney and Sue Colman 5 LDI-PCR: Identification of Known and Unknown Gene Fusions of the Human MLL Gene Claus Meyer and Rolf Marschalek 6 Panhandle PCR Approaches to Cloning MLL Genomic Breakpoint Junctions and Fusion Transcript Sequences Blaine W. Robinson and Carolyn A. Felix 7 MRD Detection in Acute Lymphoblastic Leukemia Patients Using Ig/TCR Gene Rearrangements as Targets for Real-Time Quantitative PCR Vincent H.J. van der Velden and Jacques J.M. van Dongen 8 Array-Based Comparative Genomic Hybridization as a Tool for Analyzing the Leukemia Genome Jon C. Strefford and Helen Parker 9 Application of SNP Genotype Arrays to Determine Somatic Changes in Cancer Manu Gupta and Bryan D. Young 10 Retroviral/Lentiviral Transduction and Transformation Assay Bernd B. Zeisig and Chi Wai Eric So 11 Gene Expression Profiling of Leukemia Stem Cells Andrei V. Krivtsov, Yingzi Wang, Zhaohui Feng, and Scott A. Armstrong 12 Humanized Model to Study Leukemic Stem Cells Dominique Bonnet 13 Model Systems for Examining Effects of Leukemia Associated Oncogenes in Primary Human CD34+ Cells via Retroviral Transduction Mark Wunderlich and James C.Mulloy 14 Ex-vivo Assays to Study Self-Renewal and Long-Term Expansion of Genetically Modified Primary Human Acute Myeloid Leukemia Stem Cells Jan Jacob Schuringa and Hein Schepers 15 Identification and Characterization of Hematopoietic Stem and Progenitor Cell Populations in Mouse Bone Marrow by Flow Cytometry Jenny Yeung and Chi Wai Eric So 16 In vitro Differentiation of ES Cells as a Model of Early Hematopoietic Development Patrycja Sroczynska, Christophe Lancrin, Stella Pearson, Valerie Kouskoff, and Georges Lacaud 17 Analysis of the Mouse Placenta as a Hematopoietic Stem Cell Niche Katrin Ottersbach and Elaine Dzierzak 18 Identification of Protein Interaction Partners by the Yeast Two Hybrid System Maria-Paz Garcia-Cuellar, Deniz Mederer, and Robert K. Slany 19 Complete Array of HOX Gene Expression by RQ-PCR Glenda J. McGonigle, Terence R. Lappin, and Alexander Thompson 20 Genome-Wide Determination of DNA Methylation by HpaII Tiny Fragment Enrichment by Ligation-Mediated PCR (HELP) for the Study of Acute Leukemias Maria E. Figueroa, Ari M. Melnick, and John M. Greally 21 Chromatin Immunoprecipitation (ChIP) for Analysis of Histone Modifications and Chromatin Associated Proteins Thomas A. Milne, Keji Zhao, and Jay L. Hess

Blurb

Recent advances in molecular and cellular biology techniques have significantly improved our ability to detect, monitor, model and study the underlying molecular basis and pathogenesis of leukemia, yet we are still in an early discovery stage and much more work is needed in order to develop better strategies to diagnose, classify and treat this biologically and clinically diverse disease. In Leukemia: Methods and Protocols, expert researchers bring together a wide range of state-of-the-art laboratory methods and detailed protocols that are useful for both clinical and basic research scientists working on the disease. The volume provides techniques for prenatal backtracking of leukemic clone, molecular diagnosis, detection of genome-wide genetic abnormalities and profiling, identification of unknown fusion genes, monitoring of minimal residual diseases, disease modeling using murine and human primary hematopoietic cells, studying of normal and malignant hematopoiesis, identification of interacting partners with leukemia associated oncoproteins, and global characterization of genome-wide epigenetic changes in leukemic cells. Written in the highly successful Methods in Molecular Biology™ series format, the convenient chapters contain brief introductions, lists of the necessary materials, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.

 

Comprehensive and cutting-edge, Leukemia: Methods and Protocols will help researchers to advance knowledge and have a better understanding of the disease, which will ultimately facilitate development of anti-cancer therapy and improve quality of life for patients.