Given the vital and far-reaching applications of medicinal plant metabolites worldwide, the quality and consistency of the products as well as the very survival of various species are of the utmost importance. In Protocols for In Vitro Cultures and Secondary Metabolite Analysis of Aromatic and Medicinal Plants, expert researchers provide detailed, step-by-step protocols for the establishment of in vitro cultures of key medicinal plants, their mass multiplication in a controlled environment, and step-wise secondary metabolite analysis, genetic transformation, large-scale...
Given the vital and far-reaching applications of medicinal plant metabolites worldwide, the quality and consistency of the products as well as the ...
In the dramatic and rapidly developing field of neural transplantation for CNS repair, the most powerful contributor has been the vital research focusing on stem cells. In Neural Cell Transplantation: Methods and Protocols, leading experts in the field examine tried-and-true laboratory techniques in order to supply scientists with a firm foundation upon which further advancements can be based. Written in the highly successful Methods in Molecular Biology series format, the chapters of this volume include introductions to their respective topics, lists of the necessary...
In the dramatic and rapidly developing field of neural transplantation for CNS repair, the most powerful contributor has been the vital research fo...
Expert contributors from a broad spectrum of scientific backgrounds update and expand the successful first edition with cutting-edge techniques and applications, including approaches to both antibody or B-cell epitope mapping and T-cell epitope mapping.
Expert contributors from a broad spectrum of scientific backgrounds update and expand the successful first edition with cutting-edge techniques and ap...
Over the past thirty years, the development of the Western blot has revolutionized the fields of biomedical research and medical diagnostics. In "Protein Blotting and Detection: Methods and Protocols," expert researchers present numerous techniques based on the Western blot, providing detailed, readily reproducible methods, tips, and alternatives directly and easily transferable to the laboratory setting. Chapters offer a large number of variations on the theme of protein transfer to solid support followed by detection, presenting both adaptations of traditional techniques as well as...
Over the past thirty years, the development of the Western blot has revolutionized the fields of biomedical research and medical diagnostics. In "P...
This is a comprehensive reference for developing, running, and analyzing a biomedical research system using the rat as the model. It provides practical information, a contextual discussion, and detailed descriptions of techniques used for optimal results.
This is a comprehensive reference for developing, running, and analyzing a biomedical research system using the rat as the model. It provides practica...
Now a major approach in the identification of certain gene product functions, reverse chemical genetics allows the identification of target genes to precede phenotypic changes created by specific small molecules. In Reverse Chemical Genetics: Methods and Protocols, expert authors introduce fundamental resources, including databases for reverse chemical genetics, as well as the modification of these cDNA resources by the most recent molecular technologies. The book explores newly established methods in addition to potentially applicable techniques that make use of specific small...
Now a major approach in the identification of certain gene product functions, reverse chemical genetics allows the identification of target genes t...
The feld of proteomics moves rapidly. New methods, techniques, applications, standards, models and software appear almost on a daily basis. Accompanying this are plenty of texts on the experimental side of the feld and a few appearing on the informatic and data analysis side. This latterly includes one in the Methods in Molecular Biology series tackling the specifc analysis of Mass spectrometry data in proteomics in MMB vol. 376. This current collection builds on this, but takes a broader view of proteome data analysis covering data analysis essentials, but also the databases and data models,...
The feld of proteomics moves rapidly. New methods, techniques, applications, standards, models and software appear almost on a daily basis. Accompanyi...
Antibodies tagged with fuorescent markers have been used in histochemistry for over 50 years. Although early applications were focused on the detection of microbial antigens in tissues, the use of immunocytochemical methods now has spread to include the det- tion of a wide array of antigens including proteins, carbohydrates, and lipids from virtually any organism. Today, immunohistochemistry is widely used to identify, in situ, various components of cells and tissues in both normal and pathological conditions. The method gains its strength from the extremely sensitive interaction of a specifc...
Antibodies tagged with fuorescent markers have been used in histochemistry for over 50 years. Although early applications were focused on the detectio...
Reactive oxygen and nitrogen species (RONs) affect normal physiological processes and pathological conditions. In spite of the development of analytical and technological advancements in the detection and determination of RONs, there is a growing demand to achieve more rapid and accurate detection and determination of RONs and also of redox stress. In order to satisfy such a demand, we chose to prepare this current volume which comprises 27 chapters contributed by world-renowned experts on the state-- the-art analytical and technological aspects of the detection and determination of RONs, and...
Reactive oxygen and nitrogen species (RONs) affect normal physiological processes and pathological conditions. In spite of the development of analytic...
Efforts to describe and model the molecular structure of biological membranes go back to the beginning of the last century. In 1917, Langmuir described membranes as a layer of lipids one molecule thick 1]. Eight years later, Gorter and Grendel concluded from their studies that the phospholipid molecules that formed the cell membrane were arranged in two layers to form a lipid bilayer 2]. Danielli and Robertson proposed, in 1935, a model in which the bilayer of lipids is sequestered between two monolayers of unfolded proteins 3], and the currently still accepted fuid mosaic model was...
Efforts to describe and model the molecular structure of biological membranes go back to the beginning of the last century. In 1917, Langmuir describe...
